Abstract
We investigated the distribution of four enzymes involved in the immune response of Apostichopus japonicus. We collected samples of the tentacles, papillate podium, integument, respiratory tree, and digestive tract and stained them for acid phosphatase (ACP), alkaline phosphatase (AKP), non-specific esterase (NSE) and peroxidase (POD) activity. The distribution and content of ACP, AKP, NSE, and POD differed among the tissues. The coelomic epithelium of the tentacle, papillate podium, and integument and the mucous layer of respiratory tree were positive for ACP activity. The coelomic epithelium and cuticular layer of the tentacle, papillate podium, and integument and the mucous layer and tunica externa of the respiratory tree and digestive tract stained positive or weakly positive for AKP activity. Almost all the epithelial tissues stained positive, strongly positive, or very strongly positive for NSE activity. The cuticular layer of the tentacle and integument and the mucous layer, tunica submucosa, and tunica externa of the respiratory tree and digestive tract stained positive for POD activity. We hypothesize that these enzymes play a role in the immune response in A. japonicus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Fen C E. 2001. Histochemistry. People’s Medical Publishing House Press, Beijing, China. p. 272–283. (in Chinese)
Liu Z H, Mou H J, Wang Q Y. 2003. Research progress of immune related enzymes in mollusca. Marine Fisheries Research, 24(3): 86–90
Ma Y X, Xu G R, Zhang G R, Wang E P, Chang Y Q. 2006. The etiology of acute preistome edema disease in cultured juveniles of Apostichopus japonicus. Journal of Fishery Sciences of China, 30(3): 377–382
Qiao J H, CHENG B. 2005. The situation and expection of artificial pond culture for Apostichopus joponicus (Selenlca). Marine Sciences, 29(9): 80–82.
Sun S H, Wang Y Y, Han Q, Wu M M, Kao H J. 2006. Histochemistry studies of non-specific esterase and peroxidase on embryo and early larva of oyster Crassostrea gigas. Journal of Fishery Sciences of China, 13(6): 897–901.
Wang P H, Chang Y Q, Xu G R, Song L S. 2005. Isolation and ultrastructure of an enveloped virus in cultured sea cucumber Apostichopus japonicus (Selenka). Journal of Fishery Sciences of China, 12(6): 766–771. (in Chinese with English abstract)
Wang Y Y, Sun S H, Sun X Q, Li G Y. 2004. Peroxidase activity in gill and labial palps of scallop Chlamys farreri. Journal of Fishery Sciences of China, 11(6): 513–518.
Zhang C Y, Wang Y G, Rong X J. 2006. Isolation and identification of causative pathogen for skin ulcerative syndrome in Apostichopus japonicus. Journal of Fishery Sciences of China, 30(1): 118–123. (in Chinese with English abst ract)
Author information
Authors and Affiliations
Corresponding author
Additional information
Supported by the Special Financed Project from the Basic Public Scientific Research Expenses of Central Scientific Institutes (No. GY02-2007B03)
Rights and permissions
About this article
Cite this article
Li, J., Sun, X., Zheng, F. et al. Histochemical localization and characterization of AKP, ACP, NSE, and POD from cultured Apostichopus japonicus . Chin. J. Ocean. Limnol. 27, 550–554 (2009). https://doi.org/10.1007/s00343-009-9125-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00343-009-9125-z