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Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii

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Abstract

To improve the expression efficiency of exogenous genes in Chlamydomonas reinhardtii, a high efficient expression vector was constructed. Green fluorescent protein (GFP) was expressed in C. reinhardtii under the control of promoters: RBCS2 and HSP70A-RBCS2. Efficiency of transformation and expression were compared between two transgenic algae: RBCS2 mediated strain Tran-I and HSP70A-RBCS2 mediated strain Tran-II. Results show that HSP70A-RBCS2 could improve greatly the transformation efficiency by approximately eightfold of RBCS2, and the expression efficiency of GFP in Tran-II was at least double of that in Tran-I. In addition, a threefold increase of GFP in Tran-II was induced by heat shock at 40°C. All of the results demonstrated that HSP70A-RBCS2 was more efficient than RBCS2 in expressing exogenous gene in C. reinhardtii.

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Authors and Affiliations

  1. Institute of Ecology and Environment, College of Life Sciences, Shenzhen University, Shenzhen, 518060, China

    Jinxia Wu  (吴锦霞), Zhangli Hu  (胡章立), Chaogang Wang  (王潮岗), Shuangfei Li  (黎双飞) & Anping Lei  (雷安平)

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  1. Jinxia Wu  (吴锦霞)
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  2. Zhangli Hu  (胡章立)
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  3. Chaogang Wang  (王潮岗)
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  4. Shuangfei Li  (黎双飞)
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  5. Anping Lei  (雷安平)
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Corresponding author

Correspondence to Zhangli Hu  (胡章立).

Additional information

Supported by the High Technology Research and Development Program of China (863 Program) (No. 2005AA601010-05), the Natural Science Foundation of Guangdong Province (No.5010492), and the Technology Project of Shenzhen City

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Wu, J., Hu, Z., Wang, C. et al. Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii . Chin. J. Ocean. Limnol. 26, 242–247 (2008). https://doi.org/10.1007/s00343-008-0242-x

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  • DOI: https://doi.org/10.1007/s00343-008-0242-x

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