Abstract
The pigments in Laminaria japonica was extracted with six organic solvents and analyzed in spectroscopy analysis. The extractions conditions were screened by an orthogonal test and the quantity of extracted pigments was determined spectroscopically. The results show that: (1) among the six organic solvents, acetone was the most effective one for the extraction; (2) the optimum extraction conditions were as follows: the ratio of S/M (solvent volume/ material weight) was 30 ml/g; fresh seaweed was extracted 2 times in 2 h; (3) the average total content of pigments was 1.85 mg/g (calculated with dry L. japonica).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chen, J., X. Xie, Y. Pang, H. Ding and X. Wang, 2004. Rapid calculation for the content of carotenoids in carrot. Xinjiang Agricultural Sciences p.103–105. (in Chinese)
Hayato M., M. Hosokawa, T. Sashima, K. Funayama and K. Miyashita, 2005. Fucoxanthin from edible seaweed, Undaria pinnatifida, shows antiobesity effect through UCP1 expression in white adipose tissues. Biochem. Bioph. Res. Co. 332: 392–397.
Hosokawa, M., W. Satoshi, M. Kozue, H. Kurihara, H. Kohno, J. Kawabata, S. Odashima and K. Takahashi, 1999. Apoptosis inducing effect of fucoxanthin on human leukemia cell line HL-60. Food Sci. Technol. Res. 5: 243–246.
Ji, M. H., 2004. Seaweed Chemistry. Science Press, Beijing, China, p461–477. (in Chinese)
Kim, J. M., Y. Tanaka, T. Osawa, T. Uraji, M. Murakoshi, H. Nishino and H. Tsuda, 1998. Chemopreventive effects of carotenoids and curcumins on mouse colon carcinogenesis after 1,2-dimethylhydrazine initiation. Carcinogenesis 19: 81–85.
Kotake-Nara, E., M. Kushiro, T. Sugawara, K. Miyashita and A. Nagao, 2001. Carotenoids affect proliferation of human prostate cancer cells. J. Nutr. 131: 3 303–3 306.
Nishino, H., H. Tokuda, Y. Satomi, 1999. Cancer prevention by carotenoids. Pure Appl. Chem. 71(12): 2273–2278.
Okuzumi, J., H. Nishino, M. Murakoshi, A. Iwashima, Y. Tanaka, T. Yamane, Y. Fujita and T. Takahashi, 1990. Inhibitory effects of fucoxanthin, a natural carotenoid on N-myc expression and cell cycle progression in human malignant tumor cells. Cancer Lett. 55: 75–81.
Seely, G. R., 1972. Preparative and analytical extraction of pigments from brown algae with dimethyl sulfoxide. Mar. Biol. 12: 184–188.
Author information
Authors and Affiliations
Corresponding author
Additional information
Supported by the Technical Program of Jiangsu Province (BE 2006383) and the Talent Program of Chinese Academy of Sciences (Dongbei Zhi Chun)
Rights and permissions
About this article
Cite this article
Yang, L., Li, P. & Fan, S. The extraction of pigments from fresh Laminaria japonica . Chin. J. Ocean. Limnol. 26, 193–196 (2008). https://doi.org/10.1007/s00343-008-0193-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00343-008-0193-2