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Dynamic three-wavelength imaging and volumetry of flowing cells with doubled field of view by six-pack holography

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Abstract

We present a new six-pack holography (6PH) system for simultaneous acquisition of six off-axis holograms in a single exposure from two fields of view and three wavelengths. The system is based on an external 6PH module, placed at the output of an inverted microscope, presenting a significantly more elegant solution compared to previous 6PH systems, which were based on cumbersome Mach–Zehnder interferometers built around the sample. This is also the first time in which six-pack holography is utilized for multi-wavelength imaging. We demonstrate using the new system to acquire volumetric data of flowing erythrocytes with twice the field of view of previous multi-wavelength holographic techniques. This volumetric data includes mean corpuscular volume, mean corpuscular hemoglobin (Hb) concentration, mean corpuscular Hb, and sphericity.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, 69978, Tel Aviv, Israel

    Simcha K. Mirsky & Natan T. Shaked

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  1. Simcha K. Mirsky
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  2. Natan T. Shaked
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Correspondence to Natan T. Shaked.

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Mirsky, S.K., Shaked, N.T. Dynamic three-wavelength imaging and volumetry of flowing cells with doubled field of view by six-pack holography. Appl. Phys. B 128, 92 (2022). https://doi.org/10.1007/s00340-022-07812-z

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