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Multi-color ultrafast laser platform for nonlinear optical imaging based on independently tunable optical parametric oscillators

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Abstract

We report on design of a multi-color laser set up that allows for high spectral, time and spatial resolution imaging based on second- and third-order optical nonlinearities in soft condensed matter. Two femtosecond optical parametric oscillators (OPOs) are pumped simultaneously to provide intrinsically synchronized pulses at more than a dozen tunable colors across visible and infrared wavelengths. We demonstrate the use of independently tunable OPOs in a variety of imaging modalities. In one useful application, we explore brain tissue in a two-photon absorption fluorescence imaging experiment with near infrared optical pulses (λ ~ 1,070 nm). We also demonstrate second and sum-frequency generation microscopies in different tissues. Results from application of time-resolved, three-color coherent anti-stokes Raman scattering in tissue are presented to demonstrate feasibility of quantitative spectroscopic imaging.

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Acknowledgments

Authors thank professor A. Agmon of Sensory Neuroscience Research Center for providing cortical tissue. FG acknowledges financial support from NSF CAREER award (No0952532), NSF ECCS (award No0925437) grants, and the National Institutes of Health (NIH) NIH/NIGMS CoBRE grant P30 GM103503 and ARRA Supplement S1 to the WVU Center for Neuroscience. Shan Yang’s current address is Department of Mechanical and Aerospace Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH, 44106.

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Authors and Affiliations

  1. Department of Physics, West Virginia University, PO BOX 6315, Morgantown, WV, 26506, USA

    Shan Yang, Sanjay Adhikari, Manoj Dobbala, Sasidhar Adusumilli, Joseph D. Rowley & Feruz Ganikhanov

  2. Center for Neuroscience, West Virginia University, 1 Medical Center drive, Morgantown, WV, 26506, USA

    Lingquin Zhang, Glen Marrs, Robert Wysolmerski & George Spirou

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  1. Shan Yang
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  2. Sanjay Adhikari
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Correspondence to Feruz Ganikhanov.

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Yang, S., Adhikari, S., Dobbala, M. et al. Multi-color ultrafast laser platform for nonlinear optical imaging based on independently tunable optical parametric oscillators. Appl. Phys. B 111, 617–625 (2013). https://doi.org/10.1007/s00340-013-5381-x

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