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Multifocal multiphoton microscopy based on a spatial light modulator

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Abstract

We present a new multifocal multiphoton microscope that employs a programmable spatial light modulator to generate dynamic multifocus arrays which can be rapidly scanned by changing the incident angle of the laser beam using a pair of galvo scanners. Using this microscope, we can rapidly select the number and the spatial density of focal points in a multifocus array, as well as the locations and shapes of arrays according to the features of the areas of interest in the field of view without any change to the hardware.

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Acknowledgements

This work has been partially supported by NIH (SC COBRE P20RR021949 and Career Award 1k25hl088262-01), NSF (MRI CBET-0923311 and SC EPSCoR RII EPS-0903795 through SC GEAR program), The National Natural Science Foundation of China (31171372, 60627003 and 61078067), Guangdong Province Science and Technology Project (10B060300002), HK-SZ Innovation Circle Program Project (ZYB200907090128A), Shenzhen Sci & Tech project (JC201005250032A), and Shenzhen University Application Technology Development Project (201136).

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Authors and Affiliations

  1. College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen, 518060, China

    Y. Shao, J. Qu, X. Peng & H. Niu

  2. Department of Bioengineering and COMSET, Clemson University, Clemson, SC, 29634, USA

    W. Qin, H. Liu & B. Z. Gao

Authors
  1. Y. Shao
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  2. W. Qin
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  3. H. Liu
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  4. J. Qu
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  5. X. Peng
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  6. H. Niu
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  7. B. Z. Gao
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Corresponding authors

Correspondence to J. Qu or B. Z. Gao.

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Shao, Y., Qin, W., Liu, H. et al. Multifocal multiphoton microscopy based on a spatial light modulator. Appl. Phys. B 107, 653–657 (2012). https://doi.org/10.1007/s00340-012-5027-4

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  • DOI: https://doi.org/10.1007/s00340-012-5027-4

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