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High-speed spectrally resolved multifocal multiphoton microscopy

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Abstract

We present a spectrally resolved multifocal multiphoton microscopy that is capable of performing fast 2-dimensional (2-D) spectral measurements of fluorescent samples with optical sectioning. One galvanometer mirror is used to scan the array of excitation foci across the sample along one direction (x) for two-photon excitation. Fluorescence emission from the excited lines on the sample is spectrally fanned out with a prism along the y direction, and a CCD array is used to acquire the spectrally resolved image. Another galvanometer mirror scans the excitation foci lines along the y direction step by step to obtain 3-dimensional (3-D) spectral data cube of the sample. A proof-of-principle experiment is performed with fluorescent microspheres of different colors. Spectrally resolved images of 512×512 pixels can be obtained by acquiring only 128 raw images when a 4×4 excitation foci array is used.

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Authors and Affiliations

  1. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Institute of Optoelectronics, Shenzhen University, Shenzhen, 518060, China

    Y. Shao, J. Qu, H. Li, Y. Wang, J. Qi, G. Xu & H. Niu

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  1. Y. Shao
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  2. J. Qu
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  3. H. Li
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  4. Y. Wang
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  5. J. Qi
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  6. G. Xu
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  7. H. Niu
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Correspondence to J. Qu.

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Shao, Y., Qu, J., Li, H. et al. High-speed spectrally resolved multifocal multiphoton microscopy. Appl. Phys. B 99, 633–637 (2010). https://doi.org/10.1007/s00340-010-4066-y

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  • DOI: https://doi.org/10.1007/s00340-010-4066-y

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