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Confocal mapping of myelin figures with micro-Raman spectroscopy

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Abstract

We employ confocal micro-Raman spectroscopy (CMRS) with submicron spatial resolution to study the myelin structures (cylindrical lamellae) composed of nested surfactant C12E3 or lipid DMPC bilayers. The CMRS mapping indicates that for a straight C12E3 myelin, the surfactant concentration increases with the myelin width and is higher in the center region than in the peripheral region. For a curved C12E3 myelin, the convex side has a higher surfactant concentration than the corresponding concave side. The spectrum of DMPC myelins undergoes a qualitative change as the temperature increases above 60 °C, suggesting that the surfactant molecules may be damaged. Our work demonstrates the utility of CMRS in bio-soft material research.

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Acknowledgements

The authors acknowledge financial support of the Ministry of Science and Technology of Taiwan (MOST 103-2112-M-019-003-MY3 and MOST 106-2112-M-019-005-MY3).

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Authors and Affiliations

  1. Institute of Physics, Academia Sinica, 128, Sec. 2, Academia Rd., Taipei 115, Taiwan, Republic of China

    Jung-Ren Huang & Hai-Pang Chiang

  2. Institute of Optoelectronic Sciences, National Taiwan Ocean University, 2 Pei-Ning Rd., Keelung 202, Taiwan, Republic of China

    Yu-Che Cheng & Hai-Pang Chiang

  3. Instrument Technology Research Center, National Applied Research Laboratories, 20, R&D Rd. VI, Hsinchu Science Park, Hsinchu 300, Taiwan, Republic of China

    Hung Ji Huang

  4. Physics Department, National Taiwan Normal University, 88, Sec. 4, Ting-Chou Rd., Taipei 116, Taiwan, Republic of China

    Jung-Ren Huang

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  1. Jung-Ren Huang
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  4. Hai-Pang Chiang
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Correspondence to Jung-Ren Huang or Hai-Pang Chiang.

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Huang, JR., Cheng, YC., Huang, H.J. et al. Confocal mapping of myelin figures with micro-Raman spectroscopy. Appl. Phys. A 124, 58 (2018). https://doi.org/10.1007/s00339-017-1450-z

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