Applied Physics A

, 122:1012 | Cite as

A first insight on the biodegradation of limestone: the case of the World Heritage Convent of Christ

  • Tânia Rosado
  • Mara Silva
  • Andreia Galvão
  • José Mirão
  • António Candeias
  • Ana Teresa CaldeiraEmail author
Part of the following topical collections:
  1. Innovation in Art Research and Technology


The present study is a multidisciplinary approach applied to architectural stone materials of the Convent of Christ in Tomar (Portugal) in order to understand and mitigate the active decay processes. The structure and appearance of the stonework from the Convent of Christ are strongly affected by stains, biofilms and structural degradation. To investigate these phenomena, a multianalytical approach comprising X-ray microdiffraction, scanning electron microscopy, microRaman and microinfrared spectroscopy was applied to the examination of altered outdoor stone areas being detected calcium oxalates, carotenoids and microbial proliferation. The presence of these alteration products seems to be correlated with the microbial activity of bacteria, microalgae, cyanobacteria and filamentous fungi. This work showed that the application of complementary methodologies is an efficient strategy to characterise the stone decay, and constitute a starting point for successful conservation intervention plans that are urgent to ensure the preservation and safeguard of this emblematic monument.


Carotenoid Calcium Oxalate Rose Bengal Alteration Product Stone Material 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors gratefully acknowledge HIT3CH Project “HERCULES Interface for Technology Transfer and Teaming in Cultural Heritage” (ALT20-03-0246-FEDER-000004) and MEDUSA Project “Microorganisms Monitoring and Mitigation—Developing and Unlocking Novel Sustainable Approaches” (ALT20-03-0145-FEDER-000015). Co-financed by the European Regional Development Fund (ERDF) and ALENTEJO 2020 (Alentejo Regional Operational Programme) for the financial support, and, Biotechnology and Biochemistry undergraduate students Rita Santos, Mónica Lança and Carla Nogueira for helping in the laboratory analyses.


  1. 1.
    M. Slavíková, F. Krejčí, J. Žemlička, M. Pech, P. Kotlík, J. Jakůbek, J. Cult Herit. 13, 357 (2012)CrossRefGoogle Scholar
  2. 2.
    M. Korkanç, Constr. Build. Mater. 48, 789 (2013)CrossRefGoogle Scholar
  3. 3.
    L.K. Herrera, H.A. Videla, Int. Biodeterior. Biodegradation 63, 813 (2009)CrossRefGoogle Scholar
  4. 4.
    B. Prieto, B. Silva, N. Aira, L. Álvarez, Int. Biodeterior. Biodegrad. 58, 150 (2006)CrossRefGoogle Scholar
  5. 5.
    A.M.M. Essa, M.K. Khallaf, Int. Biodeterior. Biodegrad. 94, 31 (2014)CrossRefGoogle Scholar
  6. 6.
    M. Korkanç, A. Savran, Constr. Build. Mater. 80, 279 (2015)CrossRefGoogle Scholar
  7. 7.
    T. Warscheid, J. Braams, Int. Biodeterior. Biodegrad. 46, 343 (2000)CrossRefGoogle Scholar
  8. 8.
    N. Cutler, H. Viles, Geomicrobiol. J. 27, 630 (2010)CrossRefGoogle Scholar
  9. 9.
    P. Nowicka-Krawczyk, J. Żelazna-Wieczorek, A. Otlewska, A. Koziróg, K. Rajkowska, M. Piotrowska, B. Gutarowska, A. Żydzik-Białek, Sci. Total Environ. 493, 116 (2014)CrossRefGoogle Scholar
  10. 10.
    M.L. Coutinho, A.Z. Miller, M.F. Macedo, J. Cult. Herit. 16, 759 (2015)CrossRefGoogle Scholar
  11. 11.
    M.J.T. Bento, in O Convento de Cristo em Tomar: do Infante D. Henrique às Empreitadas Manuelinas, ed. by Direcção-Geral do Património Cultural (DGPC) (Portugal, 2013)Google Scholar
  12. 12.
    K.H. Domsch, W. Gams, T.H. Anderson, Compendium of Soil Fungi, vol. 2 (Academic Press, London, 1980)Google Scholar
  13. 13.
    L. Rampazzi, A. Andreotti, I. Bonaduce, M.P. Colombini, C. Colombo, L. Toniolo, Talanta 63, 967 (2004)CrossRefGoogle Scholar
  14. 14.
    T. Rosado, M. Gil, J. Mirão, A. Candeias, A.T. Caldeira, Int. Biodeterior. Biodegrad. 85, 1 (2013)CrossRefGoogle Scholar
  15. 15.
    M. Çaliskan, Turk. J. Zool. 24, 103 (2000)Google Scholar
  16. 16.
    J.C. Merlin, Pure Appl. Chem. 57, 785 (1985)CrossRefGoogle Scholar
  17. 17.
    I. Agalidis, T. Mattioli, F. Reiss-Husson, Photosynth. Res. 62, 31 (1999)CrossRefGoogle Scholar
  18. 18.
    T. Rosado, A. Reis, J. Mirão, A. Candeias, P. Vandenabeele, A.T. Caldeira, Int. Biodeterior. Biodegrad. 94, 121 (2014)CrossRefGoogle Scholar
  19. 19.
    J.M. González, C. Saiz-Jiménez, Int. Microbiol. 8, 189 (2005)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Tânia Rosado
    • 1
  • Mara Silva
    • 1
    • 2
  • Andreia Galvão
    • 3
  • José Mirão
    • 4
  • António Candeias
    • 1
    • 2
  • Ana Teresa Caldeira
    • 1
    • 2
    Email author
  1. 1.HERCULES LaboratoryÉvora UniversityÉvoraPortugal
  2. 2.Chemistry Department, School of Sciences and TechnologyÉvora UniversityÉvoraPortugal
  3. 3.Convento de Cristo, Direção Geral do Património CulturalTomarPortugal
  4. 4.Geosciences Department, School of Sciences and TechnologyÉvora UniversityÉvoraPortugal

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