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Local stimulation of cultured myocyte cells by femtosecond laser-induced stress wave

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Abstract

When an 800 nm femtosecond laser is tightly focused into cell culture medium a stress wave is generated at the laser focal point. Since the stress wave localizes in a few tens of μm, it is possible to locally stimulate single cells in vitro. In this work, several kinds of cultured mammalian cells, HeLa, PC12, P19CL6, and C2C12, were stimulated by the stress wave and the cell growth after the stress loading with the laser irradiation was investigated. In comparison with the control conditions, cell growth after the laser irradiation was enhanced for the cells of C2C12 and P19CL6, which can differentiate into myocytes, and suppressed for PC12 and HeLa cell lines. These results suggest a possibility of cell growth enhancement due to myogenic cells response to the femtosecond laser-induced stress.

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Authors and Affiliations

  1. Institute of Biophotonics, National Yang-Ming University, Taipei, 11221, Taiwan

    Yung-En Kuo, Cheng-Chi Wu & Fu-Jen Kao

  2. Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan

    Yoichiroh Hosokawa, Yasuyo Maezawa, Kazunori Okano & Hiroshi Masuhara

  3. Department of Photonics, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan

    Fu-Jen Kao

Authors
  1. Yung-En Kuo
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  2. Cheng-Chi Wu
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  3. Yoichiroh Hosokawa
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  4. Yasuyo Maezawa
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  5. Kazunori Okano
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  6. Hiroshi Masuhara
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  7. Fu-Jen Kao
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Correspondence to Yoichiroh Hosokawa.

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Kuo, YE., Wu, CC., Hosokawa, Y. et al. Local stimulation of cultured myocyte cells by femtosecond laser-induced stress wave. Appl. Phys. A 101, 597–600 (2010). https://doi.org/10.1007/s00339-010-5927-2

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  • DOI: https://doi.org/10.1007/s00339-010-5927-2

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