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Label-free detection of charged macromolecules by using a field-effect-based sensor platform: Experiments and possible mechanisms of signal generation

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Abstract

The possibilities and limitations of a direct electrical detection of charged macromolecules using a field-effect-based sensor platform is evaluated, mainly focusing on capacitive EIS (electrolyte-insulator-semiconductor) devices. The experimentally obtained results on the detection of DNA immobilisation and hybridisation as well as the monitoring of layer-by-layer adsorbed charged polyelectrolyte (PE) multilayers have been discussed by using two basic possible mechanisms of signal generation, namely the intrinsic charge of the macromolecules and the charge redistribution within the intermolecular spaces or in the multilayer. The effects of the layer-by-layer adsorption conditions (unbuffered and pH buffer solution), and the number and polarity of charged layers on the sensor response have been systematically investigated by means of capacitance–voltage (C–V), constant–capacitance (ConCap) and impedance spectroscopy (IS) methods.

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References

  1. P. de-los-Santos-Alvarez, M.J. Lobo-Castanon, A.J. Miranda-Ordieres, P. Tunon-Blanco, Anal. Bioanal. Chem. 378, 104 (2004)

    Article  Google Scholar 

  2. E. Souteyrand, J.P. Cloarec, J.R. Martin, C. Wilson, I. Lawrence, S. Mikkelson, M.F. Lawrence, J. Phys. Chem. B 101, 2980 (1997)

    Article  Google Scholar 

  3. F. Uslu, S. Ingebrandt, D. Mayer, S. Böcker-Meffert, M. Odenthal, A. Offenhäusser, Biosens. Bioelectron. 19, 1723 (2004)

    Article  Google Scholar 

  4. Y. Han, A. Offenhäusser, S. Ingebrandt, Surf. Interf. Anal. 38, 176 (2006)

    Article  Google Scholar 

  5. F. Pouthas, C. Gentil, D. Cote, U. Bockelmann, Appl. Phys. Lett. 84, 1594 (2004)

    Article  ADS  Google Scholar 

  6. M.W. Dashiell, A.T. Kalambur, R. Leeson, K.J. Roe, J.F. Rabolt, J. Kolodzey, in: Proceedings IEEE Lester Eastman Conference, p. 259, Delaware (2002)

  7. D.-S. Kim, Y.-T. Jeong, H.-K. Lyu, H.-J. Park, H.S. Kim, J.-K. Shin, P. Choi, J.-H. Lee, G. Lim, M. Ishida, Japan. J. Appl. Phys. 42, 4111 (2003)

    Article  ADS  Google Scholar 

  8. J.-K. Shin, D.-S. Kim, H.-J. Park, G. Lim, Electroanalysis 16, 1912 (2004)

    Article  Google Scholar 

  9. D.-S. Kim, Y.-T. Jeong, H.-K. Lyu, H.-J. Park, J.-K. Shin, P. Choi, J.-H. Lee, G. Lim, Biosens. Bioelectron. 20, 69 (2004)

    Article  Google Scholar 

  10. D.-S. Kim, H.-J. Park, H.-M. Jung, J.-K. Shin, Y.-T. Jeong, P. Choi, J.-H. Lee, G. Lim, Japan. J. Appl. Phys. 43, 3855 (2004)

    Article  ADS  Google Scholar 

  11. K.Y. Park, M.S. Kim, S.Y. Choi, Biosens. Bioelectron. 20, 2111 (2005)

    Article  Google Scholar 

  12. T. Ohtake, C. Hamai, T. Uno, H. Tabata, T. Kawai, Japan. J. Appl. Phys. 43, L1137 (2004)

    Article  ADS  Google Scholar 

  13. T. Uno, T. Ohtake, H. Tabata, T. Kawai, Japan. J. Appl. Phys. 43, L1584 (2004)

    Article  ADS  Google Scholar 

  14. T. Sakata, M. Kamahori, Y. Miyahara, Mater. Sci. Eng. C 24, 827 (2004)

    Article  Google Scholar 

  15. T. Sakata, M. Kamahori, Y. Miyahara, Japan. J. Appl. Phys. 44, 2854 (2005)

    Article  ADS  Google Scholar 

  16. T. Sakata, S. Matsumoto, Y. Nakajima, Y. Miyahara, Japan. J. Appl. Phys. 44, 2860 (2005)

    Article  ADS  Google Scholar 

  17. Y. Ishige, M. Shimoda, M. Kamahori, Japan. J. Appl. Phys. 45, 3776 (2006)

    Article  ADS  Google Scholar 

  18. G. Xuan, J. Kolodzey, V. Kapoor, G. Gonye, Appl. Phys. Lett. 87, 103903 (2005)

    Article  Google Scholar 

  19. D. Goncalves, D.M.F. Prazeres, V. Chu, J.P. Conde, J. Non-Cryst. Solids 352, 2007 (2006)

    Article  Google Scholar 

  20. P. Estrela, A.G. Stewart, F. Yan, P. Migliorato, Electrochim. Acta 50, 4995 (2005)

    Article  Google Scholar 

  21. S.Q. Lud, M.G. Nikolaides, I. Haase, M. Fischer, A.R. Bausch, Chem. Phys. Chem. 7, 379 (2006)

    Google Scholar 

  22. M.G. Nikolaides, S. Rauschenbach, S. Luber, K. Buchholz, M. Tornow, G. Abstreiter, A.R. Bausch, Chem. Phys. Chem. 4, 1104 (2003)

    Google Scholar 

  23. P.A. Neff, A. Naji, C. Ecker, B. Nickel, R. Klitzing, A.R. Bausch, Macromolecules 39, 463 (2006)

    Article  Google Scholar 

  24. P. Estrela, P. Migliorato, H. Takiguchi, H. Fukushima, S. Nebashi, Biosens. Bioelectron. 20, 1580 (2005)

    Article  Google Scholar 

  25. J. Fritz, E.B. Cooper, S. Gaudet, P.K. Sorger, S.R. Manalis, Proc. Nat. Acad. Sci. 99, 14142 (2002)

    Article  ADS  Google Scholar 

  26. T. Sakata, Y. Miyahara, Biosens. Bioelectron. 21, 827 (2005)

    Article  Google Scholar 

  27. M.J. Schöning, M.H. Abouzar, Y. Han, S. Ingebrandt, A. Offenhäusser, A. Poghossian, in: Sensoren und Messsysteme (VDE, Frankfurt, 2006), pp. 443–446

  28. A. Poghossian, M.H. Abouzar, F. Amberger, D. Mayer, Y. Han, S. Ingebrandt, A. Offenhäusser, M.J. Schöning, Biosens. Bioelectron. (2006), in press

  29. T. Kassab, Y. Han, A. Poghossian, S. Ingebrandt, A. Offenhäusser, M.J. Schöning, Biomedizinische Technik 49, 1034 (2004)

    Google Scholar 

  30. M.J. Schöning, M.H. Abouzar, S. Ingebrandt, A. Offenhäusser, A. Poghossian, Mater. Res. Soc. Symp. Proc. Vol. 915, 2006, 0915-R05-04

  31. A. Poghossian, M.H. Abouzar, M. Sakkari, T. Kassab, Y. Han, S. Ingebrandt, A. Offenhäusser, M.J. Schöning, Sens. Actuators B 118, 163 (2006)

    Article  Google Scholar 

  32. A. Poghossian, A. Cherstvy, S. Ingebrandt, A. Offenhäusser, M.J. Schöning, Sens. Actuators B 111–112, 470 (2005)

    Google Scholar 

  33. D. Landheer, G. Aers, W.R. Mckinnon, M.J. Deen, J.C. Ranuarez, J. Appl. Phys. 98, 044701 (2005)

    Article  Google Scholar 

  34. L.J. Bousse, N.F. de Rooij, P. Bergveld, IEEE Trans. Electron. Dev. ED-30, 1263 (1983)

    Google Scholar 

  35. P. Bergveld, Sens. Actuators 6, 55 (1991)

    Google Scholar 

  36. A. Poghossian, M.J. Schöning, in: Enzyclopedia of Sensors, ed. by C.A. Crimes, E.C. Dickey, M.V. Pishko (American Scientific, Stevenson Ranch, CA, 2006), vol. 9, p. 463

  37. G. Shen, N. Tercero, M.A. Gaspar, B. Varughese, K. Shepard, R. Levicky, J. Am. Chem. Soc. 128, 8427 (2006)

    Article  Google Scholar 

  38. G.S. Manning, Quart. Rev. Biophys. II, 179 (1978)

  39. M.C. Pirrung, Angew. Chem. Int. Edit. 41, 1276 (2002)

    Article  Google Scholar 

  40. G. Decher, M. Eckle, J. Schmitt, B. Struth, Curr. Opin. Coll. Interf. Sci. 3, 32 (1998)

    Google Scholar 

  41. M. Schönhoff, Curr. Opin. Coll. Interf. Sci. 8, 86 (2003)

    Google Scholar 

  42. P.T. Hammond, Curr. Opin. Coll. Interf. Sci. 4, 430 (2000)

  43. V. Bosio, F. Dubreuil, G. Bogdanovic, A. Fery, Colloids Surf. A 243, 147 (2004)

    Google Scholar 

  44. R. Klitzing, H. Möhwald, Langmuir 11, 3554 (1995)

    Article  Google Scholar 

  45. D. Carriere, R. Krastev, M. Schönhoff, Langmuir 20, 11465 (2004)

    Article  Google Scholar 

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Authors and Affiliations

  1. Jülich Campus, Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, Ginsterweg 1, 52428, Jülich, Germany

    A. Poghossian, M.H. Abouzar & M.J. Schöning

  2. Institute of Bio- and Nanosystems, Research Centre Jülich, 52425, Jülich, Germany

    A. Poghossian, S. Ingebrandt, M.H. Abouzar & M.J. Schöning

Authors
  1. A. Poghossian
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  2. S. Ingebrandt
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  3. M.H. Abouzar
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  4. M.J. Schöning
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Corresponding author

Correspondence to M.J. Schöning.

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PACS

82.47.Rs; 82.80.Fk; 85.30.Tv; 87.15.Kg; 87.14.Gg

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Poghossian, A., Ingebrandt, S., Abouzar, M. et al. Label-free detection of charged macromolecules by using a field-effect-based sensor platform: Experiments and possible mechanisms of signal generation. Appl. Phys. A 87, 517–524 (2007). https://doi.org/10.1007/s00339-007-3919-7

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  • DOI: https://doi.org/10.1007/s00339-007-3919-7

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