Processing and modification of films made from recombinant spider silk proteins

Abstract

Protein films represent an interesting class of materials with various possibilities for applications. We investigated films made of two different synthetic spider silk proteins derived from the garden spider’s (Araneus diadematus) two dragline silk proteins ADF-3 and ADF-4. Protein films cast from hexafluoroisopropanol solutions displayed a predominantly α-helical secondary structure. Processing such films with potassium phosphate or methanol resulted in a transition to a β-sheet rich structure. While as-cast films could be dissolved in water, processed β-sheet rich films were water insoluble. The chemical stability of processed films depended on the amino acid sequence of the respective protein employed. As a proof of principle, fluorescent probes or enzymes were covalently attached to the film surface. The presented approach provides a basis for designing tailor-made protein films using silk proteins as scaffold, in which the film properties can be controlled by genetic engineering of the underlying silks.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Panilaitis B, Altman GH, Chen J, Jin HJ, Karageorgiou V, Kaplan DL (2003) Biomaterials 24:3079

    Article  PubMed  Google Scholar 

  2. 2.

    Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, Lu H, Richmond J, Kaplan DL (2003) Biomaterials 24:401

    Article  PubMed  Google Scholar 

  3. 3.

    Scheibel T (2004) Microb. Cell Fact. 3:14

    Article  PubMed  Google Scholar 

  4. 4.

    Zhao C, Yao J, Masuda H, Kishore R, Asakura T (2003) Biopolymers 69:253

    Article  PubMed  Google Scholar 

  5. 5.

    Um IC, Kweon HY, Park YH, Hudson S (2001) Int. J. Biol. Macromol. 29:91

    Article  PubMed  Google Scholar 

  6. 6.

    Ha SW, Tonelli AE, Hudson SM (2005) Biomacromolecules 6:1722

    Article  PubMed  Google Scholar 

  7. 7.

    Chen X, Knight DP, Shao Z, Vollrath F (2002) Biochem. J. 41:14944

    Article  Google Scholar 

  8. 8.

    Stephens JS, Fahnestock SR, Farmer RS, Kiick KL, Chase DB, Rabolt JF (2005) Biomacromolecules. 6:1405

    Article  PubMed  Google Scholar 

  9. 9.

    Sugihara A, Sugiura K, Morita H, Ninagawa T, Tubouchi K, Tobe R, Izumiya M, Horio T, Abraham NG, Ikehara S (2000) Proc. Soc. Exp. Biol. Med. 225:58

    Article  PubMed  Google Scholar 

  10. 10.

    Demura M, Takekawa T, Asakura T, Nishikawa A (1992) Biomaterials 13:276

    Article  PubMed  Google Scholar 

  11. 11.

    Zhou CZ, Confalonieri F, Medina N, Zivanovic Y, Esnault C, Yang T, Jacquet M, Janin J, Duguet M, Perasso R, Li ZG (2000) Nucleic Acids Res. 28:2413

    Article  PubMed  Google Scholar 

  12. 12.

    Huemmerich D, Helsen CW, Quedzuweit S, Oschmann J, Rudolph R, Scheibel T (2004) Biochem. J. 43:13604

    Article  Google Scholar 

  13. 13.

    Huemmerich D, Scheibel T, Vollrath F, Cohen S, Gat U, Ittah S (2004) Curr. Biol. 14:2070

    Article  PubMed  Google Scholar 

  14. 14.

    Sonnichsen FD, Van Eyk JE, Hodges RS, Sykes BD (1992) Biochem. J. 31:8790

    Article  Google Scholar 

  15. 15.

    Gast K, Siemer A, Zirwer D, Damaschun G (2001) Eur. J. Biophys. 30:273

    Article  Google Scholar 

  16. 16.

    J.M. Gosline, P.A. Guerette, C.S. Ortlepp, K.N. Savage, J. Exp. Biol. 23(202Pt):3295 (1999)

    Google Scholar 

  17. 17.

    Arcidiacono S, Mello CM, Butler M, Welsh E, Soares JW, Allen A, Ziegler D, Laue T, Chase S (2002) Macromolecules 35:1262 (2002)

    Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to T. Scheibel.

Additional information

PACS

68.55.Jk; 81.15.Lm; 87.14.Ee

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Huemmerich, D., Slotta, U. & Scheibel, T. Processing and modification of films made from recombinant spider silk proteins. Appl. Phys. A 82, 219–222 (2006). https://doi.org/10.1007/s00339-005-3428-5

Download citation

Keywords

  • Potassium Phosphate
  • HFIP
  • Silk Protein
  • Spider Silk
  • Guanidinium Thiocyanate