Skip to main content

Biological laser printing of three dimensional cellular structures

Applied Physics A volume 79, pages 1027–1030 (2004)Cite this article

Abstract

This proceeding presents experiments demonstrating that BioLPTM, Biological Laser Printing, can be used to rapidly and accurately print individual cells as well as patterns of cells in three dimensions. Human osteosarcoma cells were deposited into a biopolymer matrix, and after twenty-four hours of incubation, the printed cells remained viable and had multiplied. Multiple layers of human osteosarcoma cells were transferred into a MatrigelTM substrate, producing a three-dimensional cellular construct. Live/dead assays were performed, demonstrating that greater than 95% of the cells were viable after the being printed into the biopolymer. The ability to selectively print individual cells has potential benefits for cell selection studies and genomics and proteomics research. In addition, this technique could enable layers of tissue scaffolding to be seeded with cells at exceedingly high precision and resolution.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price includes VAT (USA)
Tax calculation will be finalised during checkout.

References

  1. G.N. Bancroft, V.I. Sikavitsas, J.V.D. Dolder, T.L. Sheffield, C.G. Ambrose, J.A. Jansen, A.G. Mikos: Proc. Nat. Acad. Sci. 99, 12600 (2002)

    ADS  Article  Google Scholar 

  2. L.E. Freed, G. Vunjak-Novakovic: Principles of Tissue Engineering, (Landes, Austin, 1997)

  3. R. Langer, J.P. Vacanti: Science 260, 920 (1993)

    ADS  Article  Google Scholar 

  4. R.M. Nerem: Ann. Biomed. Eng. 19, 529 (1991)

    Article  Google Scholar 

  5. B.J. Spargo, M.A. Testoff, T.B. Nielsen, D.A. Stenger, J.J. Hickman, A.S. Rudolph: Proc. Natl. Acad. Sci. USA 91, 11070 (1994)

    ADS  Article  Google Scholar 

  6. J.L. Wilbur, A. Kumar, H.A. Biebuyck, E. Kim, G.M. Whitesides: Nanotechnology 7, 452 (1996)

    ADS  Article  Google Scholar 

  7. R.S. Kane, S. Takayama, E. Ostuni, D.E. Ingber, G.M. Whitesides: Biomaterials 20, 2363 (1999)

    Article  Google Scholar 

  8. V. Mironov, T. Boland, T. Trusk, G. Forgacs, R.R. Markwald: Trends in Biotech. 21, 157 (2003)

    Article  Google Scholar 

  9. J.A. Barron, B.R. Ringeisen, B.J. Spargo: Thin Solid Films, accepted

  10. W.C. Wilson, Jr., T. Boland: Anat. Rec. 272A, 491 (2003)

    Article  Google Scholar 

  11. J.A. Barron, R. Rosen, J. Jones-Meehan, B.J. Spargo, S. Belkin, B.R. Ringeisen: Biosensors and Bioelectronics, accepted

  12. A. Karaiskou, I. Zergioti, C. Fotakis, M. Kapsetaki, D. Kafetzopoulos: Appl. Surf. Sci. 208–209, 245 (2003)

    Google Scholar 

  13. P.K. Wu, B.R. Ringeisen, D.B. Krizman, S.M. Hewitt, C.G. Frondoza, M. Brooks, D.M. Bubb, R.C.Y. Auyeung, H. Kim, A. Pique, J.M. Fitz-Gerald, B.J. Spargo, R.A. McGill, D.B. Chrisey: Rev. Sci. Instr. 74, 2546 (2003)

    ADS  Article  Google Scholar 

  14. D.J. Odde, M.J. Renn: Biotech. Bioeng. 67, 312 (2000)

    Article  Google Scholar 

  15. M.R. Emmert-Buck, R.F. Bonner, P.D. Smith, R.F. Chuaqui, Z. Zhuang, S.R. Goldstein, R.A. Weiss, L.A. Liotta: Science 274, 998 (1996)

    ADS  Article  Google Scholar 

  16. G. Paultauf, P.E. Dyer: Chem. Rev. 103, 487 (2003)

    Article  Google Scholar 

  17. D. Young, R.C.Y. Auyeung, A. Pique, D.B. Chrisey, D.D. Dlott: Appl. Surf. Sci. 197, 181 (2002)

    ADS  Article  Google Scholar 

  18. J.P. Mather, P.E. Roberts: Introduction to Cell and Tissue Culture (Plenum, New York, 1998)

  19. B.R. Ringeisen, D.B. Chrisey, A. Piqué, R. Modi, D. Young, M. Bucaro, J. Jones-Meehan, B.J. Spargo: Biomaterials. 23, 161 (2002)

    Article  Google Scholar 

  20. B.R. Ringeisen, H. Kim, J.A. Barron, D.B. Krizman, D.B. Chrisey, S. Jackman, R.Y.C. Auyeung, B.J. Spargo: Tissue Engineering, accepted

  21. P.K. Wu, B.R. Ringeisen, J. Callahan, M. Brooks, D.M. Bubb, H.D. Young, A. Piqué, B.J. Spargo, R.A. McGill, D.B. Chrisey: Thin Solid Films. 398, 607 (2001)

    ADS  Article  Google Scholar 

  22. B.R. Ringeisen, P.K. Wu, H. Kim, A. Piqué, R.Y.C. Auyeung, D. Young, D.B. Chrisey, D.B. Krizman: Biotech. Prog. 18, 1126 (2002)

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Chemical Dynamics and Diagnostics Branch, Chemistry Division, Naval Research Laboratory, Washington, DC, 20375, USA

    J.A. Barron, B.J. Spargo & B.R. Ringeisen

Authors
  1. J.A. Barron
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B.J. Spargo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B.R. Ringeisen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to B.R. Ringeisen.

Additional information

PACS

87.15.La; 87.18.Hf; 87.50.Hj

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Barron, J., Spargo, B. & Ringeisen, B. Biological laser printing of three dimensional cellular structures. Appl. Phys. A 79, 1027–1030 (2004). https://doi.org/10.1007/s00339-004-2620-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00339-004-2620-3

Keywords

  • Cell Selection
  • Osteosarcoma
  • Potential Benefit
  • High Precision
  • Individual Cell