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Applied Physics A

, Volume 79, Issue 4–6, pp 949–952 | Cite as

Laser direct writing of biomolecule microarrays

  • P. SerraEmail author
  • J.M. Fernández-Pradas
  • F.X. Berthet
  • M. Colina
  • J. Elvira
  • J.L. Morenza
Article

Abstract

Protein-based biosensors are highly efficient tools for protein detection and identification. The production of these devices requires the manipulation of tiny amounts of protein solutions in conditions preserving their biological properties. In this work, laser induced forward transfer (LIFT) was used for spotting an array of a purified bacterial antigen in order to check the viability of this technique for the production of protein microarrays. A pulsed Nd : YAG laser beam (355 nm wavelength, 10 ns pulse duration) was used to transfer droplets of a solution containing the Treponema pallidum 17 kDa protein antigen on a glass slide. Optical microscopy showed that a regular array of micrometric droplets could be precisely and uniformly spotted onto a solid substrate. Subsequently, it was proved that LIFT deposition of a T. pallidum 17 kDa antigen onto nylon-coated glass slides preserves its antigenic reactivity and diagnostic properties. These results support that LIFT is suitable for the production of protein microarrays and pave the way for future diagnostics applications.

Keywords

Protein Antigen Regular Array Bacterial Antigen Protein Microarrays Tiny Amount 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • P. Serra
    • 1
    Email author
  • J.M. Fernández-Pradas
    • 1
  • F.X. Berthet
    • 2
  • M. Colina
    • 1
  • J. Elvira
    • 3
  • J.L. Morenza
    • 1
  1. 1.Departament de Física Aplicada i ÒpticaUniversitat de BarcelonaBarcelonaSpain
  2. 2.Biokit SALliçà d’AmuntSpain
  3. 3.Nuevas Tecnologías Espaciales SALliçà d’AmuntSpain

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