Abstract
Our perception of reef diversity is dominated by corals, fish, and a few other groups that visibly dominate the reef surface. However, the bulk of reef biodiversity resides within the reef framework, and this cryptobiota is fundamentally important for the surface community. Sponges are abundant and conspicuous on the reef surface in productive, continental reefs, but largely vanish from surveys of the oligotrophic reefs of Oceania. However, their diversity in the cryptobiota remains poorly characterized. Here, we explore the contribution of cryptobenthic sponges to overall sponge diversity on 1750 m2 of reef habitat in Kāneʻohe Bay and Waimanalo in the island of Oʻahu, Hawaiʻi. We also assessed cryptic sponges using 15 m2 of autonomous reef monitoring structures (ARMS) deployed in this same area. We used integrative taxonomy combining morphology, COI and 28S barcoding to delineate and track species, most of which are poorly known or undescribed. We documented 186 OTUs, 150 of which are new records for the Hawaiian Islands, increasing the known sponge fauna of Kāneʻohe Bay by 3.5-fold, and that of the Hawaiian Islands by 2.5-fold. More than ¾ of the sponge OTUs were cryptobenthic. Reef sampling provided access to 31% (44 OTUs), whereas 52% (75 OTUs) were retrieved exclusively from ARMS. These results illustrate that the interstices of ARMS units provide suitable habitat for settlement of cryptobenthic sponges that would otherwise be impossible to access through traditional field surveys. Tracking species with provisional names, using integrative species delineation anchored to vouchers, images, and DNA barcodes provides a powerful approach for working with such a poorly understood fauna.
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Acknowledgements
Funding was provided through a combination of NSF Postdoctoral Research Fellowship in Biology (award no. 1612307 to JV and NSF award OA#1416889 to RJT) and by the National Oceanic and Atmospheric Administration’s Ocean Acidification Program. Additional funding was provided by the Department of Land and Natural Resources Hawaiian Invasive Species Council (FY19 Grant Award). We thank the chairs of the MarineGEO Hawai ‘i Team, M. Hagedorn, L. R. Parenti, C. Lager and M. X. Weber, the Tennenbaum Marine Observatory Network, the SI Global Genome Initiative, The Leonard P. Schultz Fund and the Herbert R. and Evelyn Axelrod Endowment, Division of Fishes, National Museum of Natural History, the Smithsonian Tropical Research Institute, the Smithsonian Conservation Biology Institute, The Castle Foundation, the Matthew Frank Foundation, the Zilber Family Foundation and the Hawaiʻi Institute of Marine Biology. These are SOEST 11342 and HIMB 1855, contribution numbers. Michael Wallstrom is thanked for his help in sponge collecting and vouchering and the members of the ToBo lab for their support and discussions.
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Vicente, J., Webb, M.K., Paulay, G. et al. Unveiling hidden sponge biodiversity within the Hawaiian reef cryptofauna. Coral Reefs 41, 727–742 (2022). https://doi.org/10.1007/s00338-021-02109-7
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DOI: https://doi.org/10.1007/s00338-021-02109-7