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Coral Reefs

, Volume 26, Issue 3, pp 531–538 | Cite as

Dark calcification and the daily rhythm of calcification in the scleractinian coral, Galaxea fascicularis

  • F. A. Al-HoraniEmail author
  • É. Tambutté
  • D. Allemand
Report

Abstract

The rate of calcification in the scleractinian coral Galaxea fascicularis was followed during the daytime using 45Ca tracer. The coral began the day with a low calcification rate, which increased over time to a maximum in the afternoon. Since the experiments were carried out under a fixed light intensity, these results suggest that an intrinsic rhythm exists in the coral such that the calcification rate is regulated during the daytime. When corals were incubated for an extended period in the dark, the calcification rate was constant for the first 4 h of incubation and then declined, until after one day of dark incubation, calcification ceased, possibly as a result of the depletion of coral energy reserves. The addition of glucose and Artemia reduced the dark calcification rate for the short duration of the experiment, indicating an expenditure of oxygen in respiration. Artificial hypoxia reduced the rate of dark calcification to about 25% compared to aerated coral samples. It is suggested that G. fascicularis obtains its oxygen needs from the surrounding seawater during the nighttime, whereas during the day time the coral exports oxygen to the seawater.

Keywords

Corals Galaxea fascicularis Calcification rhythm Dark calcification 

Notes

Acknowledgments

This study was funded in part by the Max Planck Institute for Marine Microbiology-Bremen, Germany and the Monaco Scientific Center-Monaco. We would like to thank Dr. Dirk De Beer from the MPI-Bremen and Dr. Sylvie Tambutté from the Monaco Scientific Center for their help in logistics. We are also indebted to Mr. Dominique Desgré for his help in maintenance and preparation of the coral fragments.

References

  1. Al-Horani FA, Al-Moghrabi SM, De Beer D (2003a) The mechanism of calcification and its relationship to photosynthesis and respiration in the scleractinian coral Galaxea fascicularis. Mar Biol 142:419–426Google Scholar
  2. Al-Horani FA, Al-Moghrabi SM, De Beer D (2003b) Microsensor study of photosynthesis and calcification in the scleractinian coral, Galaxea fascicularis: active internal carbon cycle. J Exp Mar Biol Ecol 288:1–15CrossRefGoogle Scholar
  3. Al-Horani FA, Ferdelman T, Al-Moghrabi SM, De Beer D (2005) Spatia distribution of calcification and photosynthesis in the scleractinian coral Galaxea fascicularis. Coral Reefs 142:419–426Google Scholar
  4. Allemand D, Tambutte E, Girard JP, Jaubert J (1998) Organic matrix synthesis in the scleractinian coral Stylophora pistillata: role in biomineralization and potential target of the organotin tributyltin. J Exp Mar Biol 201:2001–2009Google Scholar
  5. Al-Moghrabi S, Allemand D, Jaubert J (1993) Valine uptake by the scleractinian coral Galaxea fascicularis: characterization and effect of light and nutritional status. J Comp Physiol B 163:355–362CrossRefGoogle Scholar
  6. Al-Moghrabi S, Goiran C, Allemand D, Speziale N, Jaubert J (1996) Inorganic carbon uptake for photosynthesis by the symbiotic coral-dinoflagellate association II Mechanisms for bicarbonate uptake. J Exp Mar Biol Ecol 199:227–248CrossRefGoogle Scholar
  7. Barnes DJ, Chalker BE (1990) Calcification and photosynthesis in reef-building corals and algae. In: Dubinsky Z (ed) Coral reefs. Elsevier, Amsterdam, pp 109–131Google Scholar
  8. Barnes DJ, Crossland CJ (1978) Diurnal productivity and apparent 14C-calcification in the staghorn coral, Acropora acuminate. Comp Biochem Physiol A 59:133–138CrossRefGoogle Scholar
  9. Barnes DJ, Taylor DL (1973) In situ studies of calcification and photosynthetic carbon fixation in the coral Montastrea annularis. Helgol Mar Res 24:284–291Google Scholar
  10. Chalker BE, Taylor DL (1975) Light-enhanced calcification, and the role of oxidative phosphorylation in calcification of the coral Acropora cervicornis. Proc R Soc Lond B 190:323–331CrossRefGoogle Scholar
  11. Chalker BE, Taylor DL (1978) Rhythmic variations in calcification and photosynthesis associated with the coral Acropora cervicornis (Lamarck). Proc R Soc Lond B 201:179–189Google Scholar
  12. Chalker BE (1977) Daily variation in the calcification capacity of Acropora cervicornis. Proc 3rd Int Coral Reef Symp 2:417–424Google Scholar
  13. Clausen CD, Roth AA (1975) Estimation of coral growth-rates from laboratory 45Ca-incorporation rates. Mar Biol 33:85–91CrossRefGoogle Scholar
  14. De Beer D, Kühl M, Stambler N, Vaki L (2000) A microsensor study of light enhanced Ca2+ uptake and photosynthesis in the reef-building hermatypic coral Favia sp. Mar Ecol Prog Ser 194:75–85Google Scholar
  15. Dennison WC, Barnes DJ (1988) Effect of water motion on coral photosynthesis and calcification. J Exp Mar Biol Ecol 115:67–77CrossRefGoogle Scholar
  16. Drew EA (1973) The biology and physiology of alga-invertebrate symbiosis III In situ measurements of photosynthesis and calcification in some hermatypic corals. J Exp Mar Biol Ecol 13:165–179CrossRefGoogle Scholar
  17. Edmunds PJ, Davies PS (1988) Post-illumination stimulation of respiration rate in the coral Porites Porites. Coral Reefs 7:7–9CrossRefGoogle Scholar
  18. Falkowski PG, Dubinsky Z, Muscatine L, Porter JW (1984) Light and the bioenergetics of a symbiotic coral. BioScience 34:705–709CrossRefGoogle Scholar
  19. Fang LS, Chen YWJ, Chen CS (1989) Why does the white tip of stony coral grow so fast without zooxanthellae? Mar Biol 103:359–363CrossRefGoogle Scholar
  20. Ferrier-Pagès C, Gattuso JP, Cauwet G, Jaubert J, Allemand D (1998) Release of dissolved organic carbon and nitrogen by the zooxanthellate coral Galaxea fascicularis. Mar Ecol Prog Ser 172:265–274Google Scholar
  21. Ferrier-Pages C, Witting J, Tambutte E, Sebens KP (2003) Effect of natural zooplankton feeding on the tissue and skeletal growth of the scleractinian coral Stylophora pistillata. Coral Reefs 22:229–240CrossRefGoogle Scholar
  22. Frankignoulle M, Canon C, Gattuso JP (1994) Marine calcification as a source of carbon dioxide: positive feedback of increasing atmospheric CO2. Limnol Oceanogr 39:458–462CrossRefGoogle Scholar
  23. Furla P, Galgani I, Durand I, Allemand D (1998) Sources and mechanisms of inorganic carbon transport for coral calcification and photosynthesis. J Exp Biol 203:3445–3457Google Scholar
  24. Gattuso JP, Allemand D, Frankignoulle M (1999) Photosynthesis and calcification at cellular organismal and community levels in coral reefs: a review on interaction and control by carbonate chemistry. Am Zool 39:160–183Google Scholar
  25. Gattuso JP, Buddemeier RW (2000) Calcification and CO2. Nature 407:311–313CrossRefGoogle Scholar
  26. Gattuso JP, Frankignoulle M, Bourge I, Romaine S, Buddemeier RW (1998) Effect of calcium carbonate saturation of seawater on coral calcification. Global Planet Change 18:37–46CrossRefGoogle Scholar
  27. Goiran C, Al-Moghrabi S, Allemand D, Jaubert J (1996) Inorganic carbon uptake for photosynthesis by the symbiotic coral/dinoflagellate association I Photosynthetic performances of symbionts and dependence on sea water bicarbonate. J Exp Mar Biol Ecol 199:207–225CrossRefGoogle Scholar
  28. Goreau TF (1959) The physiology of skeleton formation in corals I A method for measuring the rate of calcium deposition by corals under different conditions. Biol Bull 116:59–7CrossRefGoogle Scholar
  29. Hidaka M (1991) Deposition of fusiform crystals without apparent diurnal rhythm at the growing edge of septa of the coral Galaxea fascicularis. Coral Reefs 10:41–45CrossRefGoogle Scholar
  30. Houlbrèque F, Tambutté É, Ferrier-Pages C (2003) Effect of zooplankton availability on the rates of photosynthesis, and tissue and skeletal growth in the scleractinian coral Stylophora pistillata. J Exp Mar Biol Ecol 296:145–166CrossRefGoogle Scholar
  31. Ip YK, Lim ALL, Lim RWL (1991) Some properties of calcium-activated adenosine triphosphatase from the hermatypic coral Galaxea fascicularis. Mar Biol 111:191–197CrossRefGoogle Scholar
  32. Kleypas JA, Buddemeier RW, Archer D, Gattuso JP, Langdon C, Opdyke BN (1999) Geochemical consequences of increased atmospheric carbon dioxide on coral reefs. Science 284:118–120CrossRefGoogle Scholar
  33. Kuehl M, Cohen Y, Dalsgaard T, Jorgensen BB, Revsbech NP (1995) Microenvironment and photosynthesis of zooxanthellae in scleractinian corals studied with microsensors for O2, pH and light. Mar Ecol Prog Ser 117:159–172Google Scholar
  34. Langdon C, Takahashi T, Sweeney C, Chipman D, Goddard J, Marubini F, Aceves H, Barnett H, Atkinson MJ (2000) Effect of calcium carbonate saturation state on the calcification rate of an experimental coral reef. Global Biogeochem Cycles 14:639–654CrossRefGoogle Scholar
  35. Langdon C, Atkinson MJ (2005) Effect of elevated pCO2 on photosynthesis and calcification of corals and interactions with seasonal change in temperature/irradiance and nutrient enrichment. J Geophys Res 110:C09S07 doi:10.1029/2004JC002576CrossRefGoogle Scholar
  36. Leclercq N, Gattuso JP, Jaubert J (2002) Primary production, respiration, and calcification of a coral Reef mesocosm under increased CO2 partial pressure. Limnol Oceanogr 47:558–564CrossRefGoogle Scholar
  37. Lesser MP, Weis VM, Patterson MR, Lokiel PL (1994) Effects of morphology and water motion on carbon delivery and productivity in the reef coral, Pocillopora damicornis (Linnaeus): diffusion barriers, inorganic carbon limitation, and biochemical plasticity. J Exp Mar Biol Ecol 178:153–179CrossRefGoogle Scholar
  38. Levy O, Dubinsky Z, Schneider K, Achituv Y, Zakai D, Gorbunov MY (2004) Diurnal hysteresis in coral photosynthesis. Mar Ecol Prog Ser 268:105–117CrossRefGoogle Scholar
  39. Marshall AT, Clode PL (2003) Light regulated Ca2+ uptake and O2 secretion at the surface of a scleractinian coral Galaxea fascicularis. Comp Biochem Physiol Part A Mol Integr Physiol 136:417–426CrossRefGoogle Scholar
  40. Marubini F, Barnett H, Langdon C, Atkinson MJ (2001) Dependence of calcification on light and carbonate ion concentration for the hermatypic coral Porites compressa. Mar Ecol Prog Ser 220:153–162Google Scholar
  41. Marubini F, Ferrier-Pages C, Cuif JP (2002) Suppression of growth in scleractinian corals by decreasing ambient carbonate ion concentration: a cross-family comparison. Proc R Soc Lond B 270:179–184CrossRefGoogle Scholar
  42. Moya A, Tambutté S, Tambutté É, Zoccola D, Caminiti N, Allemand D (2006) Study of calcification during a daily cycle of the coral Stylophora pistillata Implications for “light-enhanced calcification”. J Exp Biol 209:3413–3419CrossRefGoogle Scholar
  43. Muscatine L (1990) The role of symbiotic algae in carbon and energy flux in reef corals. In: Dubinsky Z (ed) Coral reefs. Elsevier, Amsterdam, pp 75–84Google Scholar
  44. Nakamura T, van Woesik R (2001) Water-flow rates and passive diffusion partially explain differential survival of corals during the 1998 bleaching event. Mar Ecol Prog Ser 212:301–304Google Scholar
  45. Patton JS, Abraham S, Benson AA (1977) Lipogenesis in the intact coral Pocillopora capitata and its isolated zooxanthellae: evidence for a light-driven carbon cycle between symbiont and host. Mar Biol 44:235–247CrossRefGoogle Scholar
  46. Pätzold J (1984) Growth rhythms recorded in stable isotopes and density bands in the reef coral Porites lobata (Cebu, Philippines). Coral Reefs 3:87–90CrossRefGoogle Scholar
  47. Pearse VB, Muscatine L (1971) Role of symbiotic algae (zooxanthellae) in coral calcification. Biol Bull 141:350–363CrossRefGoogle Scholar
  48. Reynaud S, Leclercq N, Romaine-Lioud S, Ferrier-Pages C, Jaubert J, Gattuso JP (2003) Interacting effects of CO2 partial pressure and temperature on photosynthesis and calcification in a scleractinian coral. Global Change Biol 9:1660–1668CrossRefGoogle Scholar
  49. Rinkevich B, Loya Y (1983) Oriented translocation of energy in grafted reef corals. Coral Reefs 1:243–247CrossRefGoogle Scholar
  50. Rinkevich B, Loya Y (1984) Does light enhance calcification in hermatypic corals. Mar Biol 80:1–6CrossRefGoogle Scholar
  51. Tambutté É, Allemand D, Bourge I, Gattuso JP, Jaubert J (1995) An improved 45Ca protocol for investigating physiological mechanisms in coral calcification. Mar Biol 122:453–459CrossRefGoogle Scholar
  52. Tambutté É, Allemand D, Mueller E, Jaubert J (1996) A compartmental approach to the mechanism of calcification in hermatypic corals. J Exp Biol 199:1029–1041Google Scholar
  53. Ulstrup KE, Hill R, Ralph PJ (2005) Photosynthetic impact of hypoxia on in hospite zooxanthellae in the scleractinian coral Pocillopora damicornis. Mar Ecol Prog Ser 286:125–132Google Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Marine Science StationAqaba Jordan
  2. 2.Centre Scientifique de Monacoav. St MartinMonaco
  3. 3.UMR 1112 UNSA-INRANice Cedex 2France

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