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Linkage mapping of rat Chromosome 5 markers generated from chromosome-specific libraries

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Abstract.

Seventy-six novel microsatellite markers with various simple sequence repeat (SSR) motifs are reported in this paper. They were generated on the basis of non-radioactive library screening procedures from flow-sorted rat Chromosome (Chr) 5-specific DNA, and were mapped in three rat backcross populations. Fifty-four of these markers mapped to Chr 5, while the other 22 mapped to other chromosomes of the rat genome. The marker D3Uwm8 is a new microsatellite marker for the rat syndecan 4 (ryudocan) gene. A genotyping protocol based on agarose gel electrophoresis is also provided in this paper.

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Authors and Affiliations

  1. McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison, Wisconsin, 53706, USA, , , , , , US

    Hong Lan, Laurie A. Shepel, Jill D. Haag & Michael N. Gould

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  1. Hong Lan
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  2. Laurie A. Shepel
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  3. Jill D. Haag
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  4. Michael N. Gould
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Received: 17 December 1998 / Accepted: 17 February 1999

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Lan, H., Shepel, L., Haag, J. et al. Linkage mapping of rat Chromosome 5 markers generated from chromosome-specific libraries. 10, 687–691 (1999). https://doi.org/10.1007/s003359901071

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  • DOI: https://doi.org/10.1007/s003359901071

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