Abstract
From a resource of porcine ESTs, 52 transcripts were selected for regional chromosomal assignments in a somatic cell hybrid panel. Except for six ESTs, the chosen transcripts represented genes where the BLASTX database searches showed high similarity scores (> 90%) with a part of the single pass 5′ sequence to human, bovine, mouse, or pig entries. PCR primers for hybrid cell analysis of the ESTs were positioned in the 3′ UTR of the sequences. Confident regional assignments to pig chromosomes were obtained for 33 of the 52 porcine ESTs. Comparative human mapping data were available for 27 of these. Twenty-four proved to be orthologous genes now placed on the porcine transcription map. The data presented provide further comparative data for 13 autosomes and the X chromosome.
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Chaudhary R, Winterø AK, Fredholm M, Chowdhary BP (1997) FISH mapping of seven cDNA sequences in the pig. Chromosome Res 5, 545–549
Chevalet C, Gouzy J, San Cristobal-Gaudy M (1996) Regional assignment of markers using a somatic cell hybrid panel: a WWW interactive program for the pig genome. Comput Appl Biosci 13, 69–73
Chowdhary BP, Frönicke L, Gustavsson I, Schertan H (1996) Comparative analysis of the cattle and human genomes: detection of ZOO-FISH and gene mapping-based chromosomal homologies. Mamm Genome 7, 297–302
Fridolfsson A-K, Hori T, Winterø AK, Fredholm M, Yerle M, Robic A, Andersson L, Ellegren H (1997) Expansion of the pig comparative map by expressed sequence tags (EST) mapping. Mamm Genome, 8, 907–912
Frönicke L, Chowdhary BP, Scherthan H, Gustavsson I (1996) A comparative map of the porcine and human genomes demonstrates ZOO-FISH and gene mapping-based chromosomal homologies. Mamm Genome 7, 285–290
Gish W, States DJ (1993) Identification of protein coding regions by database similarity search. Nat Genet 3, 266–272
Goureau A, Yerle M, Schmitz A, Riquet J, Milan D, Pinton P, Gellin J (1996) Human and porcine correspondence of chromosome segments using bidirectional chromosome painting. Genomics 36, 252–262
Jørgensen CB, Winterø AK, Yerle M, Fredholm M (1997) Mapping of twenty-two expressed sequence tags isolated from a porcine small intestine cDNA library. Mamm Genome 8, 423–427
Lyons LA, Laughlin TF, Copeland NG, Jenkins NA, Womack JE, O’Brien SJ (1997) Comparative anchor tagged sequences (CATS) for integrative mapping of mammalian genomes. Nat Genet 15, 47–56
Makalowski W, Zhang J, Boguski MS (1996) Comparative analysis of 1,196 orthologous mouse and human full-length mRNA and protein sequences. Genome Res 6, 846–857
Nissen PH, Winterø AK, and Fredholm M (1998) Mapping of porcine genes belonging to two different cytochrome P450 subfamilies. Anim Genet, in press
Rettenberger G, Klett C, Zechner U, Kunz J, Vogel W, Hameister H (1995) Visualization of the conservation of synteny between humans and pigs by heterologous chromosomal painting. Genomics 26, 372–378
Robic A, Riquet J, Yerle M, Milan D, Lahbib-Mansais Y, Dubut-Fontana C, Gellin J (1996) Porcine linkage and cytogenetic maps integrated by regional mapping of 100 microsatellites on somatic cell hybrid panel. Mamm Genome 7, 438–445
Rozen S, Skaletsky HJ (1996,1997) Primer3. Code available at http:// www-genome.wi.mit.edu/genome_software/other/primer3.html
Thomsen PD, Winterø AK, Fredholm M (1998) Chromosomal assignments of 19 porcine cDNA sequences by FISH. Mamm Genome, in press
Tosser-Klopp G, Mulsant P, Yerle M (1998) Regional localisations of VIM, HSD3B, ACTA1, and PGM1 in pigs. Anim Genet, in press
Wilcox AS, Khan AS, Hopkins JA, Sikela JM (1991) Use of 3′ untranslated sequences of human cDNAs for rapid chromosome assignment and conversion to STSs: implications for an expression map of the genome. Nucleic Acids Res 19, 1837–1843
Winterø AK, Fredholm M, Davies W (1996) Evaluation and characterization of a porcine small intestine cDNA library: analysis of 839 clones. Mamm Genome 7, 509–517
Yerle M, Echard G, Robic A, Mairal A, Dubut-Fontana C, Riquet J, Pinton P, Milan D, Lahbib-Mansais Y, Gellin J (1996) A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics. Cytogenet Cell Genet 73, 194–202
Yerle M, Lahbib-Mansais Y, Pinton P, Robic A, Goureau A, Milan D, Gellin G. (1997) The cytogenetic map of the domestic pig (Sus scrofa domestica). Mamm Genome 8, 592–607
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Winterø, A.K., Jørgensen, C.B., Robic, A. et al. Improvement of the porcine transcription map: localization of 33 genes, of which 24 are orthologous. Mammalian Genome 9, 366–372 (1998). https://doi.org/10.1007/s003359900771
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DOI: https://doi.org/10.1007/s003359900771