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Allele-specific PCR assays for the tub and cpe fat mutations

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References

  • Coleman DL, Eicher EM (1990) Fat (fat) and tubby (tub), two autosomal recessive mutations causing obesity syndromes in the mouse. J Hered 81, 424–427

    PubMed  CAS  Google Scholar 

  • Fricker LD, Berman YL, Leiter EH, Devi LA (1996) Carboxypeptidase E activity is deficient in mice with the fat mutation. Effect on peptide processing. J Biol Chem 271, 30619–30624

    Article  PubMed  CAS  Google Scholar 

  • Heckenlively JR, Chang B, Erway LC, Peng C, Hawes NL, Hageman GS, Roderick TH (1995) Mouse model for Usher syndrome: linkage mapping suggests homology to Usher type I reported at human chromosome 11pl5. Proc Natl Acad Sci USA 92, 11100–11104

    Article  PubMed  CAS  Google Scholar 

  • Kleyn PW, Fan W, Kovats SG, Lee JJ, Pulido JC, Wu Y, Berkenmeier LR, Misumi DJ, Holmgren L, Charlat O, Woolf EA, Tayber O, Brody T, Shu P, Hawkins F, Kennedy B, Baldini L, Ebeling C, Alperin GD, Deeds J, Lakey ND, Culpepper J, Chen H, Glüksmann-Kuis MA, Carlson GA, Duyk GM, Moore KJ (1996) Identification and characterization of the mouse obesity gene tubby: a member of a novel gene family. Cell 65, 281–290

    Article  Google Scholar 

  • Naggert JK, Fricker LD, Varlamov O, Nishina PM, Rouilla Y, Steiner DF, Carrol RJ, Paigen BJ, Leiter EH (1995) Hyperproinsulinaemia in obese fat/fat mice associated with a carboxypeptidase E mutation which reduces enzyme activity. Nature Genet 10, 135–142

    Article  PubMed  CAS  Google Scholar 

  • Noben-Trauth K, Naggert JK, North MA, Nishina PM (1996) A candidate gene for the mouse mutation tubby. Nature 380, 534–538

    Article  PubMed  CAS  Google Scholar 

  • Ohlemiller KK, Hughes RM, Mosinger-Olilvie J, Speck JD, Grosof DH, Silverman MS (1995) Cochlear and retinal degeneration in the tubby mouse. Neuroreport 6, 845–849

    Article  PubMed  CAS  Google Scholar 

  • Rust S, Funke H, Assmann G (1993) Mutagenically separated PCR (MS-PCR): a highly specific one step procedure for easy mutation detection. Nucleic Acids Res 16, 3623–3629

    Article  Google Scholar 

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Maddatu, T., Naggert, J.K. Allele-specific PCR assays for the tub and cpe fat mutations. Mammalian Genome 8, 857–858 (1997). https://doi.org/10.1007/s003359900594

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