Abstract
The human CD39-like-l gene (CD39L1) was isolated from a selected cDNA library enriched for transcripts from regions of human Chromosome (Chr) 9q. Database searches with sequences of one group of clones from the selected cDNA library showed strong amino acid homology to the lymphoid cell activation antigen CD39, an ecto-apyrase gene from human and mouse. The full-length sequence for CD39L1 identified a putative 472 amino acid protein with greater than 60% identity with the chicken muscle ecto-ATPase protein, as well as homology to a number of other known ecto-ATPases and ecto-apyrases from rat, garden pea, yeast, and Toxoplasma gondii. A high level of amino acid identity suggests that CD39L1 is closely related to the chicken muscle ecto-ATPase. The presence of the human ABC2 gene on an overlapping cosmid and hybridizations to somatic cell mapping panels suggest that CD39L1 maps to human Chr 9q34. A mouse homolog was isolated (showing greater than 78% nucleotide sequence identity) and mapped by FISH to mouse Chr 2, the syntenic region of human 9q34. The genomic structure of CD39L1 reveals 9 exons covering less than 7 kb.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Asai T, Miura S, Sibley LD, Okabayashi H, Takeuchi T (1995) Biochemical and molecular characterization of nucleoside triphosphate hydrolase isozymes from the parasitic protozoan Toxoplasma gondii. J Biol Chem 270, 11391–11397
Bermudes D, Peck KR, Afifi MA, Beckers CJM, Joiner KA (1994) Tandemly repeated genes encode nucleoside triphosphate hydrolase isoforms secreted into the parasitophorous vacuole of Toxoplasma gondii. J Biol Chem 269, 29252–29260
Breathnach R, Chambon P (1981) Organization and expression of eucaryotic split genes coding for proteins. Annu Rev Biochem 50, 349–383
Chadwick BP, Obermayr F, Frischauf A-M (1997) FKHL15, a new human member of the forkhead gene family located on chromosome 9q22. Genomics, 41, 390–396
Claros MG, Von Heijne G (1994) TopPred II: an improved software for membrane protein structure prediction. Comput Appl Biosci 10, 685–686
Di Virgilio F (1995) The P2Z purinoceptor: an intriguing role in immunity, inflammation and cell death. Immunol Today 16, 524–528
Dombrowski WE, Trevilyan JM, Cone JC, Lu Y, Phillips CA (1993) Identification and partial characterization of an ectoATPase expressed by human natural killer cells. Biochemistry 32, 6515–6522
Duensing S, Kirshner H, Atzpodien J (1994) CD39 as a novel marker of in vivo immune activation. Blood 83, 3826–3827
Felts PA, Smith KJ (1996) Changes in the distribution of a calcium-dependent ATPase during demyelination and remyelination in the central nervous system. J Neurocytol 25, 171–180
Filippini A, Taffs RE, Agui T, Sitkovsky MV (1990) Ecto-ATPase activity in cytolytic T-lymphocytes. Protection from the cytolytic effects of extracellular ATP. J Biol Chem 267, 16081–16087
Gouttefangeas C, Mansur I, Schmid M, Dastot H, Gelin C, Mahouy G, Boumsell L, Bensussan A (1992) The CD39 molecule defines distinct subsets within alloactivated human CD8-positive cells. Eur J Immunol 22, 2681–2685
Ikewaki N, Takabe S, Tsuji K (1992) Development and characterization of a human monoclonal antibody probably detecting the leukocyte differentiation antigen CD39. Tissue Antigens 39, 174–181
Jones C, Kao T (1984) Regional mapping of the folylpolyglutamate synthetase gene (FPGS) to 9cen-q34. Cytogenet Cell Genet 37, 499
Kansas G, Wood G, Tedder TF (1991) Expression, distribution, and biochemistry of human CD39. Role in activation-associated homotypic adhesion of lymphocytes. J Immunol 146, 2235–2244
Kirley TL (1997) Complementary DNA cloning and sequencing of the chicken muscle Ecto-ATPase. J Biol Chem 272, 1076–1081
Kittel A, Bacsy E (1994) Ecto-ATPases and 5′-nucleotidases in the caveolae of smooth muscle. Enzyme-histochemical evidence may indicate a role for caveolae in neurotransmission. Cell Biol Int Rep 18, 875–880
Kittel A, Miklos I, Bacsy E (1995) Heterogeneous distribution of ecto-Ca-ATPases in primary cultures of human adenohypophyseal cells. Scanning Microsc 9, 490–492
Korn B, Sedlacek Z, Manaca C, Kioschis P, Konecki D, Lehrach H, Poustka A (1992) A strategy for the selection of transcribed sequences in the Xq28 region. Hum Mol Genet 1, 235–242
Kozak M (1989) The scanning model for translation: an update. J Biol Chem 108, 229–241
Lin SH (1989a) Localization of the ecto-ATPase (ecto-nucleotidase) in the rat hepatocyte plasma membrane. Implications for the functions of the ecto-ATPase. J Biol Chem 264, 14403–14407
Luciani MF, Denizot F, Savary S, Mattei MG, Chimini G (1994) Cloning of two novel ABC transporters mapping on human chromosome 9. Genomics 21, 150–159
Maliszewski CR, Delespesse GJT, Schoenborn MA, Armitage RJ, Fanslow WC, Nakajima T, Baker E, Sutherland GR, Poindexter K, Birks C, Alpert A, Friend D, Gimpel SD, Gayle III, RB (1994) The CD39 lymphoid cell activation antigen. Molecular cloning and structural characterisation. J Immunol 153, 3574–3583
Plesner L (1995) Ecto-ATPases: identities and functions. Int Rev Cytol 158, 141–214
Povey S, Attwood J, Chadwick B, Frezal J, Haines JL, Knowles M, Benham F, Kwiatowski DJ, Olopade OI, Slaugenhaupt S, Spurr NK, Smith M, Steel K, White JA, Pericak-Vance MA (1997)Report on the fifth international workshop on chromosome 9. Ann Hum Genet, in press
Rowe M, Hildreth JEK, Rickinson AB, Epstein MA (1982) Monoclonal antibodies to Epstein-Barr virus-induced, transformation-associated cell surface antigens: binding patterns and effect upon virus-specific T-cell cytotoxicity. Int J Cancer 29, 373–381
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press)
Stout JG, Kirley TL (1994) Purification and characterization of the ecto-Mg-ATPase of chicken gizzard smooth muscle. J Biochem Biophys Methods 29, 61–75
Strobel R, Rosenberg M (1993) Immunoaffinity Chromatographic purification of chicken ectoATP diphosphohydrolase. Ann NY Acad Sci 671, 487–489
Wang TF, Guidotti G (1996) CD39 is an ecto-(Ca2+, Mg2+)-apyrase. J Biol Chem 271, 9898–9901
Author information
Authors and Affiliations
Additional information
The nucleotide sequences reported in this paper have been submitted to the GenBank™database with the accession numbers U91510 (human) and U91511 (mouse).
Rights and permissions
About this article
Cite this article
Chadwick, B.P., Frischauf, AM. Cloning and mapping of a human and mouse gene with homology to ecto-ATPase genes. Mammalian Genome 8, 668–672 (1997). https://doi.org/10.1007/s003359900534
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s003359900534