Abstract
We have isolated genomic sequences as well as transcripts from the bovine homolog of the human testis-specific protein, Y-encoded, TSPY which—in both species—is located on the Y Chromosome (Chr), organized as a gene family with a variable number of members, and expressed exclusively in the testis. 1266 bp of bovine TSPY specific sequence have been isolated from a testis cDNA library, by RT-PCR analyses and by Rapid Amplification of cDNA Ends (RACE). A bovine TSPY gene 4 is organized in seven exons, and transcripts are polyadenylated at various 3′ ends. Consensus polyadenylation signals AA UUAAA are missing. Microheterogeneous sequence variation is found between TSPY family members. In addition, homologies to other Y-located repeated sequence families, BRY, have been discovered; these sequences are presumably derived from ancient members of the TSPY cluster, now forming a separate, probably nonfunctional subfamily. Bovine TSPY is subject to differential splicing. In the adult, it is expressed in early germ-cell stages, and expression could also be detected in fetal testis. Comparison with the human homolog shows the highest degree of similarity in the coding regions of exons 2, 3, and 4, which are also precisely conserved regarding their length.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Affara N, Bishop C, Brown W, Cooke H, Davey P, Ellis N, Graves JM, Jones M, Mitchell M, Rappold G, Tyler-Smith C, Yen P, Lau Y-FC (1996) Report of the second international workshop on Y chromosome mapping 1995. Cytogenet Cell Genet 73, 33–76
Arnemann J, Jakubiczka S, Thiiring S, Schmidtke J (1991) Cloning and sequence analysis of a human Y-chromosome-derived, testicular cDNA, TSPY. Genomics 11, 108–114
Chandley AC, Cooke HJ (1994) Human male fertility-Y-linked genes and spermatogenesis. Hum Mol Genet 3, 1449–1452
Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162, 156–158
Cooper DN, Krawczak M (1993) Human Gene Mutations. (Oxford: BIOS) pp. 244–245
Cotinot C, Kirszenbaum, M, Leonard M, Gianquinto L, Vaiman M (1991) Isolation of bovine Y-derived sequence: potential use in embryo sexing. Genomics 10, 646–653
Curtis SK, Amann RP (1981) Testicular development and establishment of spermatogenesis in Holstein Bulls. J Anim Sci 53, 1645–1657
Daneau I, Houde A, Ethier J-F, Lussier JG, Silversides DW (1995) Bovine SRY gene locus: cloning and testicular expression. Biol Rep (Wash. DC) 52, 591–599
Ertl C, Wrobel K-H (1992) Ultrastructure and morphometry of prepuberal postnatal germ cell development in the bovine testis. Reprod Domest Anim 27, 73–84
Feinberg M, Vogelstein B (1984) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132, 6–13
Jakubiczka S, Schnieders F, Schmidtke J (1993) A bovine homologue of the human TSPY gene. Genomics 17, 732–735
Manz E, Schnieders F, Müller Brechlin A, Schmidtke J (1993) TSPY-related sequences represent a microheterogeneous gene family organized as constitutive elements in DYZ5 tandem repeat units on the human Y chromosome. Genomics 17, 726–731
Mathews ME, Reed K (1991) A DNA sequence that is present in both sexes of artiodactyla is repeated on the Y chromosome of cattle, sheep, and goats. Cytogenet Cell Genet 56, 40–44
Mathews ME, Reed K (1992) Sequences from a family of bovine Y-chromosomal repeats. Genomics 13, 1267–1273
Moss SB, Ferry RA, Groudine M (1994) An alternative pathway of histone mRNA 3′ end formation in mouse round spermatids. Nucleic Acids Res 22, 3160–3166
Rabbitts KG, Morgan GT (1992) Alternative 3′ processing of Xenopus α-tubulin mRNAs; efficient use of a CAUAAA polyadenylation signal. Nucleic Acids Res 20, 2947–2953
Schempp W, Binkele A, Arnemann J, Glaser B, Ma K, Taylor K, Toder R, Wolfe J, Chandley AC (1995) Comparative mapping of YRRM- and TSPY-related cosmids in man and hominoid apes. Chromosome Res 3, 227–234
Schnieders F, Jakubiczka S, Jung C, Schmidtke J (1994) Evolutionary relationships between TSPY, BRY, and BC1.2, members of a Y-chromosomal sequence family in mammals. Report and abstracts of the first international workshop on Y chromosome mapping 1994. Cytogenet Cell Genet 67, 359–402
Schnieders F, Dörk T, Arnemann J, Vogel T, Werner M, Schmidtke J (1996) TSPY expression in testicular tissues. Hum Mol Genet 5, 1801–1807
Su H, Lau T-FC (1993) Identification of the transcriptional unit, structural organization, and promotor sequence of the human sex-determining region Y (SRY) gene, using a reverse genetic approach. Am J Hum Genet 52, 24–38
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignments through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680
Tsuchiya K, Reijo R, Page D, Disteche CM (1995) Gonadoblastoma: molecular definition of the susceptibility region on the Y chromosome. Am J Hum Genet 57, 1400–1407
Tyler-Smith C, Taylor L, Müller U (1988) Structure of a hypervariable tandemly repeated DNA sequence in the short arm of the human Y chromosome. J Mol Biol 203, 837–848
Uhlig H, Saeger W, Fehr S, Lüdecke DK (1991) Detection of growth hormone, prolactin and human β-chorionic gonadotropin messenger RNA in growth-hormone-secreting pituitary adenomas by in situ hybridization. Virchows Arch Abt A Pathol Anat 418, 539–546
Wilusz J, Pettine SM, Shenk T (1989) Functional analysis of point mutations in the AAUAAA motif of the SV40 polyadenylation signal. Nucleic Acids Res 10, 3899–3908
Zhang JS, Yang-Feng TL, Müller U, Mohandas TK, de Jong PJ, and Lau Y-FC (1992) Molecular isolation and characterization of an expressed gene from the human Y chromosome. Hum Mol Genet 1, 717–726
Author information
Authors and Affiliations
Additional information
The nucleotide sequence data reported in this paper have been submitted to GenBank and have been assigned the accession numbers U75888, U75889, U75890, U75891, U75892, U75893, U75894, U75895, U75896.
Rights and permissions
About this article
Cite this article
Vogel, T., Dechend, F., Manz, E. et al. Organization and expression of bovine TSPY . Mammalian Genome 8, 491–496 (1997). https://doi.org/10.1007/s003359900482
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s003359900482