Abstract
The Hybrid sterility 1 (Hst1) gene causes male infertility in crosses between certain inbred strains of the laboratory and wild mouse, Mus musculus. To identify the causative gene, we have searched YAC clones encompassing the Hstl region for testis-expressed sequences, using the cDNA selection method. We isolated 12 non-overlapping cDNA clones, sequenced them, and placed them on a physical map based on the analysis of YAC clones and total genomic DNA. The cDNA clones map to ten loci. Three cDNA sequences correspond to the proteasome subunit C5 (locus Psmbl), ornithine decarboxylase (Odc-rs15), and pentazinc finger (Zfp91-rsl) transcripts. Three of the ten testis-expressed loci described in this report (D17Ph4e, Psmbl, and Zfp91-rs1) co-segregate with all Hst1 recombinants and, together with the Tbp gene, are therefore potential candidates for the Hst1 gene. The presented physical and genetic mapping data indicate there are no gross rearrangements distinguishing the Hst1 f and Hst1s alleles.
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The nucleotide sequence data reported in this paper have been submitted to GenBank and have been assigned the accession numbers U49252-U49257. The mapping data have been submitted to the Mouse Genome Database under the accession number MGD-CREX-656 (genetic mapping) and MGD-PMEX-16 (physical mapping).
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Trachtulec, Z., Mňuková-Fajdelová, M., Hamvas, R.M.J. et al. Isolation of candidate hybrid sterility 1 genes by cDNA selection in a 1.1 megabase pair region on mouse chromosome 17. Mammalian Genome 8, 312–316 (1997). https://doi.org/10.1007/s003359900430
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DOI: https://doi.org/10.1007/s003359900430