Abstract
The cosegregation of ten coding loci has been investigated, in a panel of 37 somatic cell hybrids resulting from the fusion of a hamster cell line and river buffalo lymphocytes, by use of Southern hybridization technique. Five syntenic groups, TCRB-PGY3, ASS-ABL, FUCA1P-CRYG, MBP-YES1, and CGN1-ACTA1, previously assigned to cattle as U13, U16, U17, U28, and U29 respectively, were also found to be syntenic in buffalo. Based on the extensive syntenic conservation and banding homology between cattle and river buffalo, comparative mapping predicts the localization of these syntenic groups on river buffalo Chromosomes (Chrs) :BBU7, BBU12, BBU2q, BBU22, and BBU4q respectively as they have been previously localized on cattle Chrs BTA4, BTA11, BTA2, BTA24 & BTA28.
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Adkison, L.R., Leung, D.W., Womack, J.E. (1988). Somatic cell mapping and restriction fragment length analysis of bovine α and β interferon gene families. Cytogenet. Cell Genet. 47, 62–65.
Barendse, W., Armitage, S.M., Kossarek, L.M., Shalom, A., Kirkpatrick, B.W., Ryan, A.M., Clayton, D., Li, L., Neibergs, H.L., Zhang, N., Grosse, W.M., Weiss, J., Creighton, P., McCarthy, F., Ron, M., Teal, A.J., Fries, R., McGraw, R.A., Moore, S.S., Georges, M., Soller, M., Womack, J.E., Hetzel D.J.S. (1994). A genetic map of the bovine genome. Nature Genet. 6, 227–235.
Blin, N., Stafford, D.W. (1976). A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res. 3, 2303–2308.
Buckland, R.A., Evans, H.J. (1978). Cytogenic aspects of phylogeny in the Bovidae. 1: G-banding. Cytogenet. Cell Genet. 21, 42–63.
Bunch, T.D., Nadler, C.F. (1980). Giemsa-band patterns of the tahr and chromosomal evolution of the tribe caprini. J. Hered. 71, 110–116.
Chevalet, C., Corpet, F. (1986). Statistical decision rules concerning synteny or independence between markers. Cytogenet. Cell Genet. 43, 132–139.
Chowdhary, R., Chowdhary, B.P., Johansson, M., Gustavsson, I. (1993). The gene for bovine interferon gamma (IFNG) maps to the q22–q24 bands of chromosome 5 in cattle. Hereditas 119, 11–14.
De Hondt, H.A., Bosma, A.A., den Bieman, M., de Haan, N.A., van Zut-phen, L.F.M. (1991). Gene mapping in river buffalo (Bubalus bubalis L.). Genet. Sel. Evol., Suppl. 1, 104s-108s.
Di Berardino, D., Iannuzzi, L. (1981). Chromosome banding homologies in Swamp and Murrah buffalo. J. Hered. 72, 183–188.
Di Berardino, D., Iannuzzi, L. (1984). Detailed description of RBA banded chromosomes of river buffalo (Bubalis bubalis L.). Genet. Sel. Evol. 16, 249–260.
El Nahas, S.M., de Hondt, A., Othman, O.S., Bosma, A., de Haan, N. (1993). Assignment of genes to chromosome 4 of the river buffalo with a panel of buffalo-hamster hybrid cells. J Anim. Breed. Genet. 110, 182–185.
Evans, HJ., Buckland, A., Samner, A.T. (1973). Chromosome homology and heterochromatin in goat, sheep, and ox studies by banding techniques. Chromosoma 42, 383–402.
Feinberg, A.P., Vogelstein, B. (1983). A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Bio-chem. 132, 6–13.
Feinberg, A.P., Vogelstein, B. (1984). Addendum: a technique for radio-labeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 137, 266–267.
Ferretti, L., Leone, P., Pilla, F., Zhang, Y., Guerin, G. (1994). Direct characterization of bovine microsatellites from cosmids: polymorphism and synteny mapping. Anim. Genet., in press.
Fries, R., Hediger, R., Stranzinger, G. (1986). Tentative chromosomal localization of the bovine major histocompatibility complex by in situ hybridization. Anim. Genet. 17, 287–294.
Fries, R., Eggen, A., Womack, J.E. (1993). The bovine genome map. Mamm. Genome 4, 405–428.
Gallagher, D.S., Womack, J.E. (1992). Chromosome conservation in the Bovidae. J. Hered. 83, 287–298.
Gallagher, D.S., Threadgill, D.W., Jackson, A.P., Pasham, P., Womack, J.E. (1991). Somatic mapping of bovine syntenic group. Cytogenet. Cell Genet. 56, 154–156.
Gallagher, D.S., Ryan, A.M., Liou, L.S., Sastry, K.N., Womack, J.E. (1993). Somatic cell mapping of conglutinin (CGN1) to cattle syntenic group U29 and fluorescence in situ localization to chromosome 28. Mamm. Genome 4, 716–719.
Gu, F., Chowdhary, B.P. Andersson, L., Harbitz, I., Gustavsson, I. (1992). Assignment of the bovine immunoglobulin gamma heavy chain (IGHG) gene to chromosome 21q24 band by in situ hybridization. Hereditas 117, 237–240.
Hassanane, M.S., Gu, F., Chowdhary, B.P., Anderson, L., Gustavsson, I. (1993). In situ hybridization mapping of the immunoglobulin gamma heavy chain (IGHG) gene to chromosome 20 1q23–q25 in river buffaloes. Hereditas 118, 285–288.
Hassanane, M.S., Chowdhary, B.P., Anderson, L., Gustavsson, I. (1994). Mapping the interferon gamma (IFNG) gene in river buffalo and swamp buffaloes by in situ hybridization. Hereditas 120, 29–33.
Hediger, R., Ansari, H.A., Stranzinger, G.F. (1991). Chromosome banding and gene localization support extensive conservation of chromosome structure between cattle and sheep. Cytogenet. Cell Genet. 57, 127–134.
Iannuzzi, L., Di Meo, G.P., Perucatti, A., Ferrara, L. (1990). A comparison of G and R-banding in cattle and river buffalo prometaphase chromosomes. Caryologia 43, 283–290.
Iannuzzi, L., Gallagher, D.S., Womack, J.E., Di Meo, G.P., Skow, L.C., Ferrara, L. (1993a). Chromosomal localization of the major histocompatibility complex in cattle and river buffalo by fluorescent in situ hybridization. Hereditas 118, 187–190.
Iannuzzi, L., Gallagher, D.S., Ryan, A.M., Di Meo, G.P., Womack, J.E. (1993b). Chromosomal localization of omega and trophoblast interferon genes in cattle and river buffalo by sequential R-banding and fluorescent in situ hybridization. Cytogenet. Cell Genet. 62, 224–227.
Iannuzzi, L., Di Meo, G.P., Ryan, A.M., Gallagher, D.S., Ferrara, L., Womack, J.E. (1994a). Localization of the bovine uridine monophosphate synthase (UMPS) gene to river buffalo chromosomes by FISH. Chromosome Res. 2, 255–256.
Iannuzzi, L., Gallagher, D.S., Liou, L.S., Di Meo, G.P., Ryan, A.M., Sastry, K.N., Womack, J.E. (1994b). Chromosomal localization of conglutinin (CGN1) gene to river buffalo by sequential RBH-banding and FISH. Hereditas 120, 283–286.
Lalley, P.A., Davisson, M.T., Graves, J.A.M., O’Brien, S.J., Womack, J.E., Roderick, T.H., Creall-Goldberg, N., Hillard, A.L., Doolittle, D.P., Rogers, J.A. (1989). Report of the committee on comparative mapping. Cytogenet. Cell Genet. 51, 503–532.
Li, L., Teale, A., Bensaid, A., Dunlap, S., Dietz, A.B., Womack, J.E. (1992). Somatic cell mapping of T-cell receptor CD3 complex and CD8 genes in cattle. Immunogenetics 36, 224–229.
Miller, J.R., Thomsen, P.D., Dixon, S.C., Tucker, E.M., Konfortov, B.A., Harbitz, I. (1992). Synteny mapping of the bovine IGHG2, CRC and IGFI genes. Anim. Genet. 23, 51–58.
Neibergs, H.L., Gallagher, D.S., Georges, M., Sargeant, L.S., Dietz, A.B., Womack, J.E. (1993). Physical mapping of inhibin B-A in domestic cattle. Mamm. Genome 4, 328–332.
Othman, O.E., Gallagher, D., de Hondt, H.A., El Nahas, S.M., Oraby, H.A., Bosma, A.A., Womack, J. (1994). Gene mapping in river buffalo: five syntenic groups. Proc. 11th European Colloquium on Cytogenetics of Domestic Animals, Frederiksberg, Denmark, Christensen, K., (ed) p. 193.
Report of the committee for the standardization of banded karyotypes of the river buffalo (1994). Standard karyotype of the river buffalo (Bubalis bubalis L., 2n = 50). Cytogenet. Cell Genet. 67, 102–113.
Ryan, A.M., Gallagher, D.S., Schober, S., Schwenger, B., Womack, J.E. (1994). Somatic cell mapping and in situ localization of the bovine uridine monophosphate synthase gene (UMPS). Mamm. Genome. 5, 46–47.
Sambrook, J., Fritsch, E.F., Maniatis, T. (1989). Molecular cloning: a laboratory manual, 2nd ed. (Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press).
Solinas Toldo, S., Mezzelani, A., Hawkins, G.A., Bishop, M.D., Olsaker, I., Mackinlay, A.G., Ferretti, L., Fries, R. (1995). Combined Q-banding and fluorescence in situ hybridization for the identification of bovine chromosomes 1 to 7. Cytogenet. Cell Genet. 69, 1–6.
Southern, E.M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517.
Threadgill, D.W., Womack, J.E. (1990). Syntenic conservation between humans and cattle. I. Human chromosome 9. Genomics 8, 22–28.
Threadgill, D.S., Threadgill, D.W., Moll, Y.D. Weiss, J.A., Zhang, N., Davey, H.W., Wildeman, A.G., Womack, J.E. (1994). Syntenic assignment of human chromosome 1 homologous loci in the bovine. Genomics 22, 626–630.
Womack, J.E. (1987). Comparative gene mapping: a valuable new tool for mammalian developmental studies. Dev. Genet. 8, 281–293.
Womack, J.E., Threadgill, D.W., Moll, Y.D., Faber, L.K., Foreman, M.L., Dietz, A.B., Tobin, T.C., Skow, L.K., Zneimer, S.M., Gallagher, D.S., Rogers, D.S. (1989). Syntenic mapping of 37 loci in cattle, chromosomal conservation with mouse and man. Cytogenet. Cell Genet. 51, 1109.
Womack, J.E., Dietz, A.B., Gallagher, D.S., Li, L., Zhang, N., Neibergs, H.L., Moll, Y.D., Ryan, A.M. (1991). Assignment of 47 additional comparative anchor loci to the bovine synteny map. Cytogenet. Cell Genet. 58, 2132.
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El Nahas, S.M., Oraby, H.A., de Hondt, H.A. et al. Synteny mapping in river buffalo. Mammalian Genome 7, 831–834 (1996). https://doi.org/10.1007/s003359900245
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DOI: https://doi.org/10.1007/s003359900245