Abstract
The surrogate light chain, composed of the VpreB and the λ-like proteins, plays a critical role in controlling the early stages of B lymphocyte development. The λ -@#@ like locus, located on the q11.2–q11.3 region of human Chromosome (Chr) 22, contains three genes (14.1 Fλ-1, and 16.1) among which only the 14.1 is functional. This gene contains three exons, whereas the others lack exon 1. We have isolated in fetal liver a transcript of the Fλ-1 gene that contains the exon 3 sequence and a long non-Ig related sequence upstream. We show that this sequence resulted from the splicing of three new exons located telomeric to the Fλ-1 gene, highly homologous to β-glucuronidase exon 11 (Chr 7), to the ABR exon 8 (Chr 17), and to an Expressed Sequence Tag (EST), respectively. We also show that this chimeric transcript is expressed in cells or tissues from various origins. This composite gene structure appears to be a new example of human genome flexibility, which can be explained by mechanisms such as exon shuffling and which results in the emergence of new transcription units inserted in regions involved in translocations.
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Schiff, C., Milili, M., Zucman-Rossi, J. et al. Composite exon structure of an unusual Ig λ-like gene located at human 22qll position. Mammalian Genome 7, 598–602 (1996). https://doi.org/10.1007/s003359900177
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DOI: https://doi.org/10.1007/s003359900177