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Regional assignment of human ESTs by whole-genome radiation hybrid mapping

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Abstract

The UK HGMP Resource Centre’s collection of human partial cDNA sequences (ESTs) have been examined for suitability for mapping by PCR on a panel of somatic cell hybrids. The chromosomal assignments of 92 ESTs were determined with a monochromosomal hybrid panel, and a subset of 45 were linked to genetic markers with a panel of whole-genome radiation hybrids (WG-RHs). These results demonstrate the potential of WG-RHs to construct a transcript map of the human genome.

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Hayes, P.D., Schmitt, K., Jones, H.B. et al. Regional assignment of human ESTs by whole-genome radiation hybrid mapping. Mammalian Genome 7, 446–450 (1996). https://doi.org/10.1007/s003359900130

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  • DOI: https://doi.org/10.1007/s003359900130

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