Abstract
We have cloned a porcine gene, designated TBP10, that belongs to the Tat-binding protein/26S protease subunit family. The genomic structure of the porcine TBP10 gene was analyzed after isolation of three overlapping genomic phage lambda clones. The TBP10 gene harbors 12 exons spanning 4.5 kb of chromosomal DNA. The TBP10 gene was assigned to Chromosome (Chr) 12 by fluorescence in situ hybridization (FISH) on metaphase chromosomes. The chromosomal location was confirmed by PCR analysis of a porcine-rodent hybrid cell panel. The TBP10 protein is encoded by a 1221 nucleotide cDNA and has a molecular mass of 45.6 kDa. The predicted amino acid sequence has highest similarity to the human and bovine p45 subunit of the 26S protease and the human transcription factor TRIP1. Further similarities were detected to the slime mold protein DdTBP10 and the Schizosaccharomyces pombe and Saccharomyces cerevisiae protein SUG1. Like DdTBP10 and other members of the protein family, the porcine TBP10 harbors a leucine zipper motif in the N-terminal region and a domain characteristics of ATP-dependent proteases in the C-terminal region.
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Leeb, T., Rettenberger, G., Bruch, J. et al. The porcine gene TBP10 encodes a protein homologous to the human Tat-binding protein/26S protease subunit family. Mammalian Genome 7, 180–185 (1996). https://doi.org/10.1007/s003359900050
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DOI: https://doi.org/10.1007/s003359900050