Abstract
The RN locus in pigs has a major effect on the amount of stored glycogen in white muscle and affects meat quality. The fully dominant RN − allele, associated with high glycogen content, occurs in the Hampshire breed. We have mapped the RN locus using a large half-sib family comprising one heterozygous RN − / rn + Hampshire boar mated to homozygous rn +/rn + Swedish Landrace × Swedish Yorkshire sows. The segregation at the RN locus was inferred from data on glycolytic potential and residual glycogen in white muscle which both showed clear bimodal distributions. Highly significant evidence for genetic linkage was obtained against microsatellite markers on Chromosome (Chr) 15. Multipoint analysis revealed the order Sw1111- 8.0 -S0088- 10.6 -RN- 4.8 -Sw936,Sw906 (recombination estimates are given as Kosambi cM). Comparative mapping data imply that the human homolog of RN is located on Chr 2q.
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References
Archibald, A.L., et al. (1995). The PigMaP Consortium linkage map of the pig (Sus scrofa). Mamm. Genome 6, 157–175.
Ellegren, H., Chowdhary, B.P., Johansson, M., Marklund, L., Fredholm, M., Gustavsson, I., Andersson, L. (1994). A primary linkage map of the porcine genome reveals a low rate of genetic recombination. Genetics 137, 1089–1100.
Estrade, M., Vignon, X., Monin, G. (1993a). Effect of the RN- gene on ultrastructure and protein fractions in pig muscle. Meat Sci. 35, 313–319.
Estrade, M., Vignon, X., Rock, E., Monin, G. (1993b). Glycogen hyper-accumulation in the white muscle fibres of RN- carrier pigs. A biochemical and ultrastructural study. Comp. Biochem. Physiol. 104B(2), 321–326.
Estrade, M., Ayoub, S., Talmant, A., Monin, G. (1994). Enzyme activities of glycogen metabolism and mitochondrial characteristics in muscle of RN- carrier pigs (Sus scrofa domesticus). Comp. Biochem. Physiol. 108B(3), 295–301.
Gahne, B., Juneja, R.K. (1985). Prediction of the Halothane (Hal) geno- types of pigs by deducing Hal, Phi, Po2, Pgd haplotypes of parents and offspring: results from a large-scale practice in Swedish breeds. Anim. Blood Groups Biochem. Genet. 16, 265–283.
Green, P., Falls, K., Crooks, S. (1990). Documentation for CRI-MAP, version 2.4. St. Louis: Washington University School of Medicine.
Johansson, M., Ellegren, H., Andersson, L. (1995). Comparative mapping reveals extensive linkage conservation—but with gene order rearrangements—between the pig and the human genomes. Genomics 25, 682–690.
Juneja, R.K., Gahne, B. (1987). Simultaneous phenotyping of pig plasma α-protease inhibitors (PI1, PO1A, PO1B, PI2) and four other proteins (PO2, TF, CP, HPX) by a simple method of 2D horizontal electrophoresis. Anim. Genet. 18, 197–211.
Le Roy, P., Naveau, J., Elsen, J.M., Sellier, P. (1990). Evidence for a new major gene influencing meat quality in pigs. Genet. Res. 55, 33–40.
Milan, D., Le Roy, P., Woloszyn, N., Caritez, J.C., Elsen, J.M., Sellier, P., Gellin, J. (1995). The RN locus for meat quality maps to pig chromosome 15. Genet. Sel. Evol. (Paris), in press.
Monin, G., Sellier, P. (1985). Pork of low technological quality with a normal rate of muscle pH fall in the immediate post-mortem period: the case of the Hampshire breed. Meat Sci. 13, 49–63.
Naveau, J., Pommeret, P., Lechaux, P. (1985). Proposition d’ une méthode de mesure du rendement technologique: ‘ la méthode NAPOLE’. Techni-porc 8, 7–13.
O’ Brien, S.J., ed. (1993). Genetic Maps, 6th ed. (Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press).
Rettenberger, G., Klett, C., Zechner, U., Kunz, J., Vogel, W., Hameister, H. (1995). Visualization of the conservation of synteny between pigs and humans by heterologous chromosomal painting. Genomics 26, 372–378.
Rohrer, G.A., Leeson, J.A., Keele, J.W., Smith, T.P., Beattie, C.W. (1994). A microsatellite linkage map of the porcine genome. Genetics 136, 231–245.
Talmant, A., Fernandez, X., Sellier, P., Monin, G. (1989). Glycolytic potential in Longissimus dorsi muscle of Large White pigs, as measured after in vivo sampling. Proc. 35th International Congress of Meat Science and Technology, Copenhagen, pp. 1129–1132.
Thomsen, P.D., Qvist, H., Marklund, L., Andersson, L., Sjöström, H., Norén, O. (1993). Assignment of the dipeptidylpeptidase IV (DPP4) gene to pig Chromosome 15q21. Mamm. Genome 4, 604–607.
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Mariani, P., Lundström, K., Gustafsson, U. et al. A major locus (RN) affecting muscle glycogen content is located on pig Chromosome 15. Mammalian Genome 7, 52–54 (1996). https://doi.org/10.1007/s003359900012
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DOI: https://doi.org/10.1007/s003359900012