New polymorphic markers in the vicinity of the pearl locus on mouse Chromosome 13


We have used a Mus domesticus/ -Mus spretus congenic animal that was selected for retention of Mus spretus DNA around the pearl locus to create a highly polymorphic region suitable for screening new markers. Representation difference analysis (RDA) was performed with either DNA from the congenic animal or C57BL/6J as the driver for subtraction. Four clones were identified, characterized, and converted to PCR-based polymorphic markers. Three of the four markers equally subdivide a 10-cM interval containing the pearl locus, with the fourth located centromeric to it. These markers have been placed on the mouse genetic map by use of an interspecific backcross panel between Mus domesticus (C57BL/6J) and Mus spretus generated by The Jackson Laboratory.

This is a preview of subscription content, access via your institution.


  1. Avner, P., Amar, L., Dandolo, L., Guénet, J.L. (1988). Genetic analysis of the mouse using interspecific crosses, Trends Genet. Rev. 4, 18–23.

    Article  CAS  Google Scholar 

  2. Biggin, M.D., Gibson, T.J., Hong, G.F. (1983). Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc. Natl. Acad. Sci. USA 80, 3963–3965.

    PubMed  Article  CAS  Google Scholar 

  3. Davis, L.G., Dibner, M.D., Battey, J.F. (1986). Basic Methods in Molecular Biology. (New York, N.Y.: Elsevier Science Publishers).

    Google Scholar 

  4. Dietrich, W., Katz, H., Lincoln, S.E., Shin, H.-S., Friedman, J., Dracopoli, N.C., Lander, E.S. (1992). A genetic map of the mouse suitable for typing intraspecific crosses. Genetics 131, 423–447.

    PubMed  CAS  Google Scholar 

  5. Elliot, R.W., Daniel, W.L., Taylor, B.A., Novak, E.K. (1985). Linkage of loci affecting a murine liver protein and arylsulfatase B to chromosome 13. J. Hered. 76, 243–246.

    Google Scholar 

  6. Justice, M.J., Stephenson, D.A. (1993). Mouse Chromosome 13. Mamm. Genome 4 (suppl.), S192–202.

    Article  Google Scholar 

  7. Lisitsyn, N., Lisitsyn, N., Wigler, M. (1993). Cloning the differences between two complex genomes. Science 259, 946–951.

    PubMed  Article  CAS  Google Scholar 

  8. Rikke, B.A., Pinto, L.H., Gorin, M.B., Hardies, S.C. (1993). Mus spretus-specific LINE-1 DNA probes applied to the cloning of the murine pearl locus. Genomics 15, 291–296.

    PubMed  Article  CAS  Google Scholar 

  9. Rowe, L., Nadeau, J., Frankel, W., Letts, V., Eppig, J., Ko, M., Thurston, S., Birkenmeier, E. (1994). Maps from two interspecific backcross DNA panels available as a community genetic mapping resource. Mamm. Genome 5, 253–274.

    PubMed  Article  CAS  Google Scholar 

  10. Sambrook, J., Fritsch, E.F., Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd ed. (Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press).

    Google Scholar 

  11. Sanger, F., Nicklen, S., Coulsen, A.R. (1977). DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463–5467.

    PubMed  Article  CAS  Google Scholar 

Download references

Author information



Corresponding authors

Correspondence to H. -P. Xu or B. L. Yanak or M. H. Wigler or M. B. Gorin.

Additional information

The nucleotide sequence data for clones reported in this paper have been submitted to GenBank and have been assigned the following accession numbers: U25821 (D13Gor2), U25824 (D13Gor3), U25825 (D13Gor4), and U25827 (D13Gorl).

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Xu, H.P., Yanak, B.L., Wigler, M.H. et al. New polymorphic markers in the vicinity of the pearl locus on mouse Chromosome 13. Mammalian Genome 7, 16–19 (1996).

Download citation


  • Representational Difference Analysis
  • Congenic Line
  • Backcross Panel
  • Congenic Animal
  • Restriction Fragment Length Variation