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Knockdown of UCA1 attenuated the progression of alcoholic fatty disease by sponging miR-214

Mammalian Genome volume 33, pages 534–542 (2022)Cite this article

Abstract

Alcoholic fatty liver (AFL) is the initial manifestation of Alcoholic liver disease which can develop into alcoholic cirrhosis even extensive necrosis of liver cells, which induces liver failure finally. This study aims to focus on the role of long noncoding RNA UCA1 in AFL and further explored possible mechanism of this disease. We first downloaded GSE28619 to identify the expression of UCA1 in patients with AFL and use lncRNAs microarray to confirm UCA1 expression in serum of patients with AFL. Then we established ethanol-induced L02 cell model to mimic hepatocyte injury condition. By conducting qRT-PCR, we measured the expression of LncRNA UCA1 and miR-214 in serum of patients and ethanol-induced L02 cell. MTT assay, transwell migration, ELISA, qRT-PCR, and western blotting analysis were applied to evaluating the effect of UCA1 on ethanol-induced L02 cell. The bioinformatics analysis and the rescue experiment were devoted to the underlying mechanism. In this study, we first detected the expression of UCA1 was up-regulated in serum of patients with AFL and ethanol-induced L02 cells. And knockdown of UCA1 reversed the inhibiting effect of ethanol on the biological behavior of L02 cells including cell proliferation, migration, and apoptosis. Besides, lncRNA UCA1 regulated the expression of KLF5 by sponging miR-214. LncRNA UCA1 regulated the biological behavior of ethanol-induced L02 cells by sponging miR-214, which may provide novel therapeutic strategies for alcoholic fatty liver.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (N0.81860431), National Natural Science Foundation of China (N0. 82060447) and Key research and development project of Jiangxi Province (20203BBGL73200)

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Authors and Affiliations

  1. Department of Health Management and Physical Examination, Maternal and Child Health Hospital of Nanchang University, Nanchang, 330006, Jiangxi Province, China

    Huali Xiang

  2. Department of Neurosurgery, The Cancer Affiliated Hospital of Nanchang University, Nanchang, 330029, Jiangxi Province, China

    Binfeng Tu

  3. Department of General Surgery, Children’s Hospital of Nanchang University, Nanchang, 330006, Jiangxi Province, China

    Ming Luo

  4. Department of General Surgery, The Second Affiliated Hospital of Nanchang University, No. 1, Minde Road, Nanchang, 330006, Jiangxi Province, China

    Ping Hou, Jiakun Wang, Rongguiyi Zhang & Linquan Wu

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  1. Huali Xiang
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  2. Binfeng Tu
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  5. Jiakun Wang
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  7. Linquan Wu
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Correspondence to Linquan Wu.

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Xiang, H., Tu, B., Luo, M. et al. Knockdown of UCA1 attenuated the progression of alcoholic fatty disease by sponging miR-214. Mamm Genome 33, 534–542 (2022). https://doi.org/10.1007/s00335-022-09953-0

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  • DOI: https://doi.org/10.1007/s00335-022-09953-0