Abstract
TLR8-AS1 has been characterized as an oncogenic lncRNA in ovarian cancer, while its role in hepatocellular carcinoma (HCC) is unknown. This study aimed to explore the role of TLR8-AS1 in HCC. TLR8-AS1 expression in HCC and paired non-tumor tissues from 62 HCC patients was determined by RT-qPCR. The prognostic value of TLR8-AS1 for HCC was analyzed by performing a 5-year follow-up. Correlations between TLR8-AS1 and mature miR-34a and miR-34a precursor were analyzed by Pearson’s correlation coefficient. The roles of TLR8-AS1 and miR-34a in regulating the proliferation and migration were explored by CCK-8 assay and Transwell migration assay. We found that TLR8-AS1 was upregulated in HCC and predicted poor survival. Across HCC tissues, TLR8-AS1 was inversely correlated with mature miR-34a, but not miR-34a precursor. In HCC cells, TLR8-AS1 overexpression downregulated mature miR-34a, but not miR-34a precursor. Cell proliferation and Transwell migration assay showed that TLR8-AS1 overexpression reduced the enhancing effects of miR-34a on cell proliferation and migration. TLR8-AS1 may suppress miR-34a maturation in HCC to suppress cell proliferation and migration.
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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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29 June 2023
This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1007/s00335-023-10004-5
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XS, HC, and JPZ: study concepts, study design, literature research, experimental studies, manuscript preparation and editing; WW, BZY, and ZKY: definition of intellectual content, literature research experimental studies, manuscript preparation and editing; all authors read and approved the final manuscript.
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The Ethics Committee of the First Hospital of Hebei Medical University approved this study. The work described here has been carried out in accordance with the World Medical Association (Declaration of Helsinki) for experiments involving humans. Written informed consent was obtained from all patients.
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Shi, X., Chen, H., Wang, W. et al. RETRACTED ARTICLE: LncRNA TLR8-AS1 suppresses miR-34a maturation in hepatocellular carcinoma to suppress cell proliferation and migration. Mamm Genome 32, 530–536 (2021). https://doi.org/10.1007/s00335-021-09920-1
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DOI: https://doi.org/10.1007/s00335-021-09920-1