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Circular RNA circNRIP1 plays oncogenic roles in the progression of osteosarcoma

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Abstract

Osteosarcoma (OS) is the most common malignant bone tumor in children and adolescents. Increasing evidence suggests that aberrant expression of circRNAs is associated with the occurrence and progression of many cancers. Here, we investigated the role of circNRIP1 in osteosarcoma and explored its possible underlying mechanisms. Three pairs of osteosarcoma tissues and adjacent normal tissues were applied to the detection of altered expression of circRNAs through circRNAs microarray. And the level of circNRIP1 expression was elevated in osteosarcoma tissues. Compared with that in adjacent normal tissue, circNRIP1 expression level was obviously elevated in 100 osteosarcoma tissues. Besides, circNRIP1 knockdown inhibited proliferation and migration, promoted apoptosis of osteosarcoma cells. Bioinformatic analysis demonstrated circNRIP1 contributed to FOXC2 expression by sponging miR-199a. Furthermore, METTL3 elevated circNRIP1 expression level via m6A modification. In short, METTL3-induced circNRIP1 exerted an oncogenic role in osteosarcoma by sponging miR-199a, which may provide new ideas for the treatment of osteosarcoma.

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Data availability

The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by Hong Hui Hospital.

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Authors and Affiliations

  1. Department of Spine Surgery, Hong Hui Hospital, Xi‘an Jiaotong University, Xi‘an, 710054, China

    Yibin Meng, DingJun Hao, YunFei Huang, ShuaiJun Jia, JiaNan Zhang & XiRui He

  2. Department of Orthopaedic, Hong Hui Hospital, Xi‘an Jiaotong University, No 555.Youyi East Road, Xi‘an, 710054, China

    Deyin Liu & Liang Sun

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  1. Yibin Meng
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  2. DingJun Hao
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  3. YunFei Huang
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  4. ShuaiJun Jia
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  5. JiaNan Zhang
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  6. XiRui He
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  7. Deyin Liu
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  8. Liang Sun
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Contributions

YM and DJH designed experiments; YFH, SJJ and JNZ performed experiments; XRH and DL analyzed the data; LS wrote the manuscript. All authors read and approved the manuscript.

Corresponding authors

Correspondence to Deyin Liu or Liang Sun.

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Conflict of interests

Yibin Meng, DingJun Hao, YunFei Huang, ShuaiJun Jia, JiaNan Zhang, XiRui He, Deyin Liu, and Liang Sun declare that they have no conflict of interest.

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This study was approved by the ethics committee of Hong Hui Hospital.

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All patients gave informed consent.

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Meng, Y., Hao, D., Huang, Y. et al. Circular RNA circNRIP1 plays oncogenic roles in the progression of osteosarcoma. Mamm Genome 32, 448–456 (2021). https://doi.org/10.1007/s00335-021-09891-3

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