Abstract
Atherosclerosis was an important pathophysiological basis of atherothrombotic stroke, and phosphodiesterase 4D (PDE4D) polymorphism (SNP83/rs966221) was reported to be associated with the susceptibility to atherothrombotic stroke. Aim of the present study was to explore the potential association between SNP83 and carotid atherosclerosis (CAS). 204 southern Chinese Han participants were divided into two groups according to the carotid intima-media thickness (IMT) of the carotid artery: CAS group (carotid IMT ≥ 1.0 mm) and non-CAS group (carotid IMT < 1.0 mm). Carotid IMT was measured by color Doppler ultrasound. The PDE4D SNP83 polymorphism was determined by SNaPshot technique. Our study found that SNP83 was associated significantly with CAS susceptibility under the dominant, overdominant and codominant models. After adjusting for age, gender, low-density lipoprotein cholesterol, Hemoglobin A1c, cigarette smoking, hypertension history, and diabetes mellitus history, the association still remained significant (dominant model: crude OR = 2.373, 95% CI: 1.268–4.442, P = 0.007; adjusted OR = 3.129, 95% CI: 1.104–8.866, P = 0.032; overdominant model: crude OR = 1.968, 95% CI: 1.043–3.714, P = 0.037; adjusted OR = 2.854, 95% CI: 1.005–8.108, P = 0.049; codominant: crude OR = 2.102, 95% CI: 1.110–3.979, P = 0.023; adjusted OR = 2.984, 95% CI: 1.047–8.502, P = 0.041). Carotid IMT of carriers with CT + CC genotypes was higher than carriers with TT genotype (P = 0.016). Our results indicated that the SNP83/rs966221 located on PDE4D gene was significantly associated between CAS susceptibility and carotid IMT independently of conventional risk factors in a southern Chinese Han population.
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Acknowledgements
We would like to thank all participants who agreed to participate in the study, the grants from Natural Science Foundation of Guangxi Province (2013GXNSFBA019131, 2015GXNSFAA139171, 2020GXNSFAA259053), Guangxi Scholarship Fund of Guangxi Education Department and National Natural Science Foundation of China for Young Scientists Fund (81701142).
Funding
This work was supported by the grants from Natural Science Foundation of Guangxi Province (2013GXNSFBA019131, 2015GXNSFAA139171, 2020GXNSFAA259053), Guangxi Scholarship Fund of Guangxi Education Department and National Natural Science Foundation of China for Young Scientists Fund (81701142).
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Man Luo and Jiao-xing Li designed the study and revised the manuscript. Liu-yu Liu and Dong-can Mo performed experiments, conducted data statistics and drafted the manuscript. Jian-li Li, Yi-ying Jiang, Guo-qiu Zhou, Dong-dong Jiang Li-jie Chen and Xiao-ju Wu assisted in the experiment and the collection of data. All authors read and approved the final manuscript.
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Liu, Ly., Mo, Dc., Li, Jl. et al. Associations between SNP83 of phosphodiesterase 4D gene and carotid atherosclerosis in a southern Chinese Han population: a case–control study. Mamm Genome 32, 115–122 (2021). https://doi.org/10.1007/s00335-021-09857-5
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DOI: https://doi.org/10.1007/s00335-021-09857-5