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Long-term experiment to study the development, interaction, and influencing factors of DEXA parameters

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Abstract

Dual-energy X-ray absorption (DEXA) is commonly used to measure bone mineral density (BMD), bone mineral content (BMC), and body composition data (fat mass and lean mass) for phenotype assessment in mice. We were interested in the long-term development of BMD, BMC, lean mass, and fat mass of mice, also taking into account sex and genetic background. The dataset was used to analyze correlations among the different parameters. We analyzed males and females from inbred strains C3HeB/FeJ and C57BL/6J, starting from 42 until 528 days of age. To evaluate the effect of husbandry systems, we repeated a part of the study in a second facility with a different caging system. We also assessed different DEXA settings and repeatability of the scans. The results of this study were used to draw conclusions for the use of DEXA analysis in mouse phenotyping approaches.

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Acknowledgments

We thank Reinhard Seeliger, Susanne Wittich, and Gregor Pahnke for their technical assistance. In addition, we wish to express our thanks to Monika Denk and Horst Wenig, as well as the entire GMC animal caretaker team, for their exceptional animal care. This work was supported by Infrafrontier BMBF (Grant no. 01KX1012).

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Authors and Affiliations

  1. Helmholtz Zentrum München, German Mouse Clinic, Institute of Experimental Genetics, German Research Center for Environmental Health (GmbH), Ingolstädter Landstr. 1, 85764, Neuherberg, Germany

    Helmut Fuchs, Christine Gau, Wolfgang Hans, Valerie Gailus-Durner & Martin Hrabě de Angelis

  2. Center of Life and Food Sciences Weihenstephan, Technische Universität München, Alte Akademie 8, 85354, Freising, Germany

    Martin Hrabě de Angelis

  3. German Center for Diabetes Research (DZD), Ingolstädter Landstr. 1, 85764, Neuherberg, Germany

    Martin Hrabě de Angelis

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  1. Helmut Fuchs
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  2. Christine Gau
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  3. Wolfgang Hans
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  4. Valerie Gailus-Durner
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  5. Martin Hrabě de Angelis
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Correspondence to Martin Hrabě de Angelis.

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Fuchs, H., Gau, C., Hans, W. et al. Long-term experiment to study the development, interaction, and influencing factors of DEXA parameters. Mamm Genome 24, 376–388 (2013). https://doi.org/10.1007/s00335-013-9477-8

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