Skip to main content

NIH Mouse Metabolic Phenotyping Centers: the power of centralized phenotyping

Abstract

The Mouse Metabolic Phenotyping Centers (MMPCs) were founded in 2001 by the National Institutes of Health (NIH) to advance biomedical research by providing the scientific community with standardized, high-quality phenotyping services for mouse models of diabetes, obesity, and their complications. The intent is to allow researchers to take optimum advantage of the many new mouse models produced in labs and in high-throughput public efforts. The six MMPCs are located at universities around the country and perform complex metabolic tests in intact mice and hormone and analyte assays in tissues on a fee-for-service basis. Testing is subsidized by the NIH in order to reduce the barriers for mouse researchers. Although data derived from these tests belong to the researcher submitting mice or tissues, these data are archived after publication in a public database run by the MMPC Coordinating and Bioinformatics Unit. It is hoped that data from experiments performed in many mouse models of metabolic diseases, using standard protocols, will be useful in understanding the nature of these complex disorders. The current areas of expertise include energy balance and body composition, insulin action and secretion, whole-body and tissue carbohydrate and lipid metabolism, cardiovascular and renal function, and metabolic pathway kinetics. In addition to providing services, the MMPC staff provides expertise and advice to researchers, and works to develop and refine test protocols to best meet the community’s needs in light of current scientific developments. Test technology is disseminated by publications and through annual courses.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2

References

  • Ayala JE, Bracy DP, McGuinness OP, Wasserman DH (2006) Considerations in the design of hyperinsulinemic-euglycemic clamps in the conscious mouse. Diabetes 55(2):390–397

    PubMed  Article  CAS  Google Scholar 

  • Ayala JE, Samuel VT, Morton GJ, Obici S, Croniger CM, Shulman GI et al (2010) Standard operating procedures for describing and performing metabolic tests of glucose homeostasis in mice. Dis Model Mech 3(9–10):525–534

    PubMed  Article  CAS  Google Scholar 

  • Ayala JE, Bracy DP, Malabanan C, James FD, Ansari T, Fueger PT et al (2011) Hyperinsulinemic–euglycemic clamps in conscious, unrestrained mice. J Vis Exp 57:e3188

    Google Scholar 

  • Berglund ED, Li CY, Poffenberger G, Ayala JE, Fueger PT, Willis SE et al (2008) Glucose metabolism in vivo in four commonly used inbred mouse strains. Diabetes 57(7):1790–1799

    PubMed  Article  CAS  Google Scholar 

  • Burgess SC, Jeffrey FM, Storey C, Milde A, Hausler N, Merritt ME et al (2005) Effect of murine strain on metabolic pathways of glucose production after brief or prolonged fasting. Am J Physiol Endocrinol Metab 289(1):E53–E61

    PubMed  Article  CAS  Google Scholar 

  • Ellacott KL, Morton GJ, Woods SC, Tso P, Schwartz MW (2010) Assessment of feeding behavior in laboratory mice. Cell Metab 12:10–17

    PubMed  Article  CAS  Google Scholar 

  • Jandacek RJ, Heubi JE, Tso P (2004) A novel, noninvasive method for the measurement of intestinal fat absorption. Gastroenterology 127(1):139–144

    PubMed  Article  CAS  Google Scholar 

  • Kaiyala KJ, Schwartz MW (2011) Toward a more complete (and less controversial) understanding of energy expenditure and its role in obesity pathogenesis. Diabetes 60(1):17–23

    PubMed  Article  CAS  Google Scholar 

  • Kaiyala KJ, Morton GJ, Leroux BG, Ogimoto K, Wisse B, Schwartz MW (2010) Identification of body fat mass as a major determinant of metabolic rate in mice. Diabetes 59(7):1657–1666

    PubMed  Article  CAS  Google Scholar 

  • McGuinness OP, Ayala JE, Laughlin MR, Wasserman DH (2009) NIH experiment in centralized mouse phenotyping: the Vanderbilt experience and recommendations for evaluating glucose homeostasis in the mouse. Am J Physiol Endocrinol Metab 297(4):E849–E855

    PubMed  Article  CAS  Google Scholar 

  • Qi Z, Fujita H, Jin J, Davis LS, Wang Y, Fogo AB et al (2005) Characterization of susceptibility of inbred mouse strains to diabetic nephropathy. Diabetes 54(9):2628–2637

    PubMed  Article  CAS  Google Scholar 

  • Rottman JN, Ni G, Khoo M, Wang Z, Zhang W, Anderson ME et al (2003) Temporal changes in ventricular function assessed echocardiographically in conscious and anesthetized mice. J Am Soc Echocardiogr 16(11):1150–1157

    PubMed  Article  Google Scholar 

  • Rottman JN, Ni G, Brown M (2007) Echocardiographic evaluation of ventricular function in mice. Echocardiography 24(1):83–89

    PubMed  Article  Google Scholar 

  • Shearer J, Coenen KR, Pencek RR, Swift LL, Wasserman DH, Rottman JN (2008) Long chain fatty acid uptake in vivo: comparison of [125I]-BMIPP and [3H]-bromopalmitate. Lipids 43(8):703–711

    PubMed  Article  CAS  Google Scholar 

  • Tong J, Tschöp MH, Aulinger BA, Davis HW, Yang Q, Liu J, Gaylinn BD, Thorner MO, D’Alessio D, Tso P (2010) The intestinal lymph fistula model–a novel approach to study ghrelin secretion. Am J Gastrointest Liver Physiol 298(3):G474–G480

    Article  CAS  Google Scholar 

  • Yin DP, Gao Q, Ma LL, Yan W, Williams PE, McGuinness OP et al (2011) Assessment of different bariatric surgeries in the treatment of obesity and insulin resistance in mice. Ann Surg 254(1):73–82

    PubMed  Article  Google Scholar 

Download references

Acknowledgments

The MMPCs are supported by U24 Grants DK059630, DK059632, DK059635, DK59637, DK076126, DK076169, DK076174, DK092993, and DK093000.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Maren R. Laughlin.

Additional information

This study was conducted on behalf of the Mouse Metabolic Phenotyping Centers Consortium.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Laughlin, M.R., Lloyd, K.C.K., Cline, G.W. et al. NIH Mouse Metabolic Phenotyping Centers: the power of centralized phenotyping. Mamm Genome 23, 623–631 (2012). https://doi.org/10.1007/s00335-012-9425-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00335-012-9425-z

Keywords

  • Phenotyping Test
  • Conscious Mouse
  • Mutant Mouse Line
  • International Mouse Phenotyping Consortium
  • Broad Scientific Community