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Overview of new developments in and the future of cryopreservation in the laboratory mouse

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Abstract

The large-scale mutagenesis programmes underway around the world are generating thousands of novel GA mouse strains that need to be securely archived. In parallel with advances in mutagenesis, the procedures used to cryopreserve mouse stocks are being continually refined in order to keep pace with demand. Moreover, the construction of extensive research infrastructures for systematic phenotyping is fuelling demand for these novel strains of mice and new approaches to the distribution of frozen and unfrozen embryos and gametes are being developed in order to reduce the dependency on the transportation of live mice. This article highlights some contemporary techniques used to archive, rederive, and transport mouse strains around the world.

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Authors and Affiliations

  1. Mary Lyon Centre, Medical Research Council, Harwell Science and Innovation Campus, Oxfordshire, OX11 0RD, UK

    Mo Guan, Amanda R. Pickard & Martin D. Fray

  2. Institute of Experimental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstrasse 1, 85764, Neuherberg, Germany

    Susan Marschall

  3. Istituto di Biologia Cellulare e Neurobiologia, Consiglio Nazionale delle Ricerche (CNR-EMMA), Campus “Adriano Buzzati-Traverso”, Via E. Ramarini 32, 00015, Monterotondo Scalo, Italy

    Marcello Raspa

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  1. Mo Guan
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  2. Susan Marschall
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  3. Marcello Raspa
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  4. Amanda R. Pickard
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  5. Martin D. Fray
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Correspondence to Martin D. Fray.

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Guan, M., Marschall, S., Raspa, M. et al. Overview of new developments in and the future of cryopreservation in the laboratory mouse. Mamm Genome 23, 572–579 (2012). https://doi.org/10.1007/s00335-012-9423-1

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  • DOI: https://doi.org/10.1007/s00335-012-9423-1

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