Abstract
BLAST analysis of the rat genome revealed the presence of 16 pseudogenes of isoform 1 of the mitochondrial voltage-dependent anion channel (VDAC1). Based on their structural characterization, it was concluded that these pseudogenes were formed by integration of VDAC1 cDNA into the genome, and subsequent rearrangements/mutations. By RT-PCR analysis using carefully designed primers that could not amplify the cDNA of genuine VDAC1, 8 of these 16 pseudogenes showed slight expression in certain tissues, but none of them seemed to encode a functional protein.
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Y. Ido and T. Yamamoto contributed equally to this work.
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Ido, Y., Yamamoto, T., Yoshitomi, T. et al. Pseudogenes of rat VDAC1: 16 gene segments in the rat genome show structural similarities with the cDNA encoding rat VDAC1, with 8 slightly expressed in certain tissues. Mamm Genome 23, 286–293 (2012). https://doi.org/10.1007/s00335-011-9375-x
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DOI: https://doi.org/10.1007/s00335-011-9375-x