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Polymorphisms in Ly6 genes in Msq1 encoding susceptibility to mouse adenovirus type 1

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Abstract

Strain-specific differences in susceptibility to mouse adenovirus type 1 (MAV-1) are linked to the quantitative trait locus Msq1 on mouse chromosome 15. This region contains 14 Ly6 or Ly6-related genes, many of which are known to be expressed on the surface of immune cells, suggesting a possible role in host defense. We analyzed these genes for polymorphisms between MAV-1-susceptible and MAV-1-resistant inbred mouse strains. Sequencing of cDNAs identified 12 coding-region polymorphisms in 2010109I03Rik, Ly6e, Ly6a, Ly6c1, and Ly6c2, six of which were nonsynonymous and five of which were previously unlisted in dbSNP Build 132. We also clarified sequence discrepancies in GenBank for the coding regions of I830127L07Rik and Ly6g. Additionally, Southern blotting revealed size polymorphisms within the DNA regions of Ly6e, Ly6a, and Ly6g. Collectively, these genetic variations have implications for the structure, function, and/or expression of Ly6 and Ly6-related genes that may contribute to the observed strain-specific differences in susceptibility to MAV-1.

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Acknowledgments

We thank Dave Burke, Tien-Huei Hsu, and Mike Imperiale for critical comments on the manuscript. We thank Jason Weinberg for helpful discussions. We are grateful to Shanna Ashley for technical assistance. This work was supported by National Institutes of Health grant R01 AI068645 to KRS.

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  1. Matthew T. Stier

    Present address: Vanderbilt University School of Medicine, Nashville, TN, 37232, USA

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  1. Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, 48109-5620, USA

    Matthew T. Stier & Katherine R. Spindler

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  1. Matthew T. Stier
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  2. Katherine R. Spindler
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Correspondence to Katherine R. Spindler.

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Stier, M.T., Spindler, K.R. Polymorphisms in Ly6 genes in Msq1 encoding susceptibility to mouse adenovirus type 1. Mamm Genome 23, 250–258 (2012). https://doi.org/10.1007/s00335-011-9368-9

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  • DOI: https://doi.org/10.1007/s00335-011-9368-9

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