Abstract
Mammalian epidermis is a stratified epithelium that serves as a barrier protecting the organism from mechanical stress and dehydration. Previous studies have demonstrated the importance of the actin cytoskeleton in the establishment of a functional skin epithelium. Despite what is known about the actin cytoskeleton in epithelial sheet formation, the molecules important for controlling the actin cytoskeleton during epidermal development have not been determined. Serum response factor (SRF) is a transcription factor that is considered to be an important regulator of the actin cytoskeleton. To examine the role of SRF in the developing mouse epidermis, we have employed gene targeting to ablate Srf in keratinocytes. Conditional inactivation of Srf during the embryonic timepoint leads to a defect in the organization of the epidermis. Immunohistochemical analyses demonstrated a marked loss of the filamentous actin cytoskeleton and E-cadherin localization in epidermis, as well as an aberration in the localization of tight junction proteins. Moreover, impairment of the “inside-out” epidermal barrier was shown. Srf conditional knockout keratinocytes are unable to establish proper intercellular connections or form an epithelial sheet as shown by histological examination and induced keratinocyte differentiation experiments. Our results demonstrate that Srf is essential for the actin-mediated sealing of epithelial cell-cell contacts and the development of functional stratified skin epithelium in vivo.
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Acknowledgments
This work was supported by a grant from National Institutes of Health (NIH; R01 EY016108). Support for AMV was partially provided by the NIH Predoctoral Training Program in Genetics (T32 GM07133). The authors thank Satoshi Kinoshita for generating frozen sections and the University of Wisconsin-Madison Genetics Confocal Facility for the use of the confocal microscope.
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Verdoni, A.M., Ikeda, S. & Ikeda, A. Serum response factor is essential for the proper development of skin epithelium. Mamm Genome 21, 64–76 (2010). https://doi.org/10.1007/s00335-009-9245-y
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DOI: https://doi.org/10.1007/s00335-009-9245-y