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Divergent patterns of breakpoint reuse in Muroid rodents

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Abstract

Multiple Genome Rearrangement (MGR) analysis was used to define the trajectory and pattern of chromosome rearrangement within muroid rodents. MGR was applied using 107 chromosome homologies between Mus, Rattus, Peromyscus, the muroid sister taxon Cricetulus griseus, and Sciurus carolinensis as a non-Muroidea outgroup, with specific attention paid to breakpoint reuse and centromere evolution. This analysis revealed a high level of chromosome breakpoint conservation between Rattus and Peromyscus and indicated that the chromosomes of Mus are highly derived. This analysis identified several conserved evolutionary breakpoints that have been reused multiple times during karyotypic evolution in rodents. Our data demonstrate a high level of reuse of breakpoints among muroid rodents, further supporting the “Fragile Breakage Model” of chromosome evolution. We provide the first analysis of rodent centromeres with respect to evolutionary breakpoints. By analyzing closely related rodent species we were able to clarify muroid rodent karyotypic evolution. We were also able to derive several high-resolution ancestral karyotypes and identify rearrangements specific to various stages of Muroidea evolution. These data were useful in further characterizing lineage-specific modes of chromosome evolution.

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Acknowledgments

EEM, CO, MJO, and RJO were supported by NIH grant P40-RR14279 and the UCONN Research Foundation. Thanks to Glenn Tesler for supplying the MGR program and Gianni Ferreri for running our analyses.

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Authors and Affiliations

  1. Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, 06269, USA

    E. E. Mlynarski, C. J. Obergfell & M. J. O’Neill

  2. Department of Molecular and Cell Biology, University of Connecticut, 354 Mansfield Road, Room 323, U-2131, Storrs, CT, 06269, USA

    R. J. O’Neill

Authors
  1. E. E. Mlynarski
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  2. C. J. Obergfell
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  3. M. J. O’Neill
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  4. R. J. O’Neill
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Correspondence to R. J. O’Neill.

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Mlynarski, E.E., Obergfell, C.J., O’Neill, M.J. et al. Divergent patterns of breakpoint reuse in Muroid rodents. Mamm Genome 21, 77–87 (2010). https://doi.org/10.1007/s00335-009-9242-1

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