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The Begain gene marks the centromeric boundary of the imprinted region on mouse chromosome 12

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Abstract

Although the central portion of the imprinted region on mouse chromosome 12 has been intensively analysed in the past, little is known about the neighbouring centromeric genes. A DNA sequence comparison shows that the region upstream of Dlk1 and Gtl2 is dominated by an expanded cluster of repetitive elements in the mouse. These elements separate the paternally expressed Dlk1 gene from the centromeric Begain gene. Despite the long physical distance to the IG-DMR imprinting centre, Begain is subjected to genomic imprinting. Similar to the ovine Begain gene, the homologous mouse gene encodes two different transcript variants, one of which shows a strong bias toward paternal transcription. Nevertheless, imprinting effects do not spread further centromeric to the Wdr25 gene, which is biallelically expressed as the previously studied neighbouring Wars and Yy1 genes.

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Acknowledgments

We are grateful to Anne C. Ferguson-Smith (University of Cambridge, UK) for providing tissue samples and DNA of IG-DMR knockout mice and mice carrying uniparental disomies of chromosome 12. We thank Carole Charlier (University of Liege, Belgium) for communicating unpublished data on the Begain analysis in sheep. We thank Jörn Walter for fruitful discussions, Christina Lo Porto for DNA sequencing, and Eva-Maria Dilly and Sven Oliger for animal care. We are thankful to Ulrike Fischer, Eckart Meese, Stephanie Barth, Friedrich Grässer, Carolin Jalal, and Hans Stahl (Saarland University) for providing the human cell lines. This work was supported by the Deutsche Forschungsgemeinschaft DFG (grant Nos. WA1029/3-2 and PA750/2-3), the EU FP6 Network of Excellence “The Epigenome,” and the Biotechnology and Biological Sciences Research Council (BBSRC).

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Author notes

  1. Gilles Gasparoni

    Present address: FR 2.17 Psychiatrie/Neurobiologie, Klinik für Psychiatrie und Psychotherapie, Universität des Saarlandes, Kirrberger Straße, Homburg, 66421, Germany

  2. Neil Youngson

    Present address: Epigenetics Laboratory, Queensland Institute of Medical Research, 300 Herston Road, Herston, Brisbane, QLD, 4006, Australia

Authors and Affiliations

  1. FR 8.3 Biowissenschaften, Genetik/Epigenetik, Universität des Saarlandes, Postfach 151150, Saarbrücken, 66041, Germany

    Sascha Tierling, Gilles Gasparoni & Martina Paulsen

  2. Department of Anatomy, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK

    Neil Youngson

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  1. Sascha Tierling
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  2. Gilles Gasparoni
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  4. Martina Paulsen
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Correspondence to Martina Paulsen.

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Tierling, S., Gasparoni, G., Youngson, N. et al. The Begain gene marks the centromeric boundary of the imprinted region on mouse chromosome 12. Mamm Genome 20, 699–710 (2009). https://doi.org/10.1007/s00335-009-9205-6

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  • DOI: https://doi.org/10.1007/s00335-009-9205-6

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