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SNPs in the bovine IL-10 receptor are associated with somatic cell score in Canadian dairy bulls

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Abstract

Altering the balance between pro- and anti-inflammatory responses can influence an animal’s susceptibility to acute or chronic inflammatory disease; bovine mastitis is no exception. Genetic variation in the form of single nucleotide polymorphisms (SNPs) may alter the function and expression of genes that regulate inflammation, making them important candidates for defining an animal’s risk of developing acute or chronic mastitis. The objective of the present study was to identify SNPs in genes that regulate anti-inflammatory responses and test their association with estimated breeding values (EBVs) for somatic cell score (SCS), a trait highly correlated with the incidence of mastitis. These genes included bovine interleukin-10 (IL-10) and its receptor (IL-10R), and transforming growth factor β1 (TGF-β1) and its receptor (TGF-βR). Sequencing-pooled DNA allowed for the identification of SNPs in IL-10 (n = 2), IL-10Rα (n = 6) and β (n = 2), and TGF-β1 (n = 1). These SNPs were subsequently genotyped in a cohort of Holstein (n = 500), Jersey (n = 83), and Guernsey (n = 50) bulls. Linear regression analysis identified significant SNP effects for IL-10Rα 1185C>T with SCS. Haplotype IL-10Rα AAT showed a significant effect on increasing SCS compared to the most common haplotype. The results presented here indicate that SNPs in IL-10Rα may contribute to variation in the SCS of dairy cattle. Although functional studies are necessary to ascertain whether these SNPs are causal polymorphisms or merely in linkage with the true causal SNP(s), a selection program incorporating these markers could have a beneficial influence on the average SCS and productivity of a dairy herd.

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Acknowledgments

We acknowledge the generous funding from the Canadian Dairy Commission, the Natural Science and Engineering Research Council, and represented by the Canadian Bovine Mastitis Research Network: Alberta Milk, Dairy Farmers of New Brunswick, Nova Scotia, Ontario, and Prince Edward Island, Novalait Inc., Dairy Farmers of Canada, Canadian Dairy Network, AAFC, PHAC, Technology PEI Inc., Université de Montréal, and University of Prince Edward Island. We also thank the Semex Alliance for providing semen samples for this study, and Mehdi Sargolzaei and Jalal Fatehi for providing EBVs and pedigree information on the bull population.

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  1. Department of Animal and Poultry Science, Centre for Genetic Improvement of Livestock, University of Guelph, Guelph, N1G 2W1, ON, Canada

    Chris P. Verschoor, Sameer D. Pant, Flavio S. Schenkel, Bhawani S. Sharma & Niel A. Karrow

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  1. Chris P. Verschoor
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  2. Sameer D. Pant
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  3. Flavio S. Schenkel
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Correspondence to Niel A. Karrow.

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Verschoor, C.P., Pant, S.D., Schenkel, F.S. et al. SNPs in the bovine IL-10 receptor are associated with somatic cell score in Canadian dairy bulls. Mamm Genome 20, 447–454 (2009). https://doi.org/10.1007/s00335-009-9198-1

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  • DOI: https://doi.org/10.1007/s00335-009-9198-1

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