Abstract
Previous studies on the fourth inversion of the t complex, In17(4), suggest that loci near the center of this inversion have been subjected to segmental recombination during the past 1–2 million years. We have used a combination of PCR-based restriction site (PBR) analysis and DNA sequencing to perform a high-resolution analysis of a 2-million base pair (Mbp) segment in the middle of In17(4). We examined 21 restriction sites that are polymorphic between t haplotypes and their wild-type homologs, over nine distinct loci. In addition, we examined several other polymorphic sites through DNA sequence analysis of two of these nine loci. We analyzed several haplotypes in this way, including the “complete” t haplotypes t w2, t 0, t w32, t w71, and t w75. We show that only t w32 is a true “complete” t haplotype; the remaining four t haplotypes have segments of wild-type DNA ranging from less than 100 bp to 2 Mbp. The sizes of these wild-type DNA segments are consistent with their being generated by gene-conversion events. The 2-Mbp segment is located in a region that may contain the t-complex distorter gene Tcd2. One of the nine loci examined in this study is Fgd2, a gene that has been proposed to encode Tcd2. Sequencing and PBR data show that at least a portion of the Fgd2 gene has been converted to the wild-type within t w71 and t w75mice.
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Acknowledgments
This research was supported by NIH MBRS/SCORE grant GM08043 to MAE, and by a Chicago State University Biological Sciences Department assistantship to LTW in 2002 and 2003. The authors gratefully acknowledge the helpful comments on the manuscript by Dr. Joyce Ache Gana.
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Wallace, L.T., Erhart, M.A. Recombination within mouse t haplotypes has replaced significant segments of t-specific DNA. Mamm Genome 19, 263–271 (2008). https://doi.org/10.1007/s00335-008-9103-3
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DOI: https://doi.org/10.1007/s00335-008-9103-3