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Genotype of bovine sterol regulatory element binding protein-1 (SREBP-1) is associated with fatty acid composition in Japanese Black cattle

Mammalian Genome volume 18, pages 880–886 (2007)Cite this article

Abstract

To investigate genetic factors that affect fatty acid composition in beef carcass, we previously investigated genetic profiles of stearoyl-CoA desaturase (SCD) and their effect on fatty acid composition in fat tissue of cattle. It has been known that sterol regulatory element binding protein (SREBP) is a transcription factor that regulates gene expression levels of SCD and other genes relevant to lipid and fatty acid metabolism in tissue. Therefore, we determined the full-length sequence of bovine SREBP-1 cDNA and then surveyed polymorphisms in whole exons and introns in the bovine genome. Large 84-bp insertion (long type: L) and deletion (short type: S) were found in intron 5 of bovine SREBP-1 in Japanese Black cattle, although there was no notable mutation in exon regions. The associations between the SREBP-1 genotypes and fatty acid compositions/fat melting points were analyzed by using genomic DNA with carcass trait information from 606 Japanese Black cattle. The S type contributed to 1.3% higher monounsaturated fatty acid (MUFA) proportion and 1.6°C lower melting point in intramuscular fat. Genotyping of bovine SREBP-1 is considered to reflect a genetic variation which is associated with physiologic characteristics of fat tissue in Japanese black cattle.

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Acknowledgments

The authors thank Livestock Improvement Association of Japan Inc. for providing us with samples and data for their progeny testing.

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Affiliations

  1. Laboratory of Animal Breeding & Genetics, Graduate School of Agricultural Science, Kobe University, Nada, Kobe, 657-8501, Japan

    Shogo Hoashi, Nobuhisa Ashida, Hideki Ohsaki, Takeshi Utsugi, Shinji Sasazaki, Fumio Mukai & Hideyuki Mannen

  2. Department of Agricultural, Food and Nutritional Science, University of Alberta, T6G 2P5, Edmonton, Alberta, Canada

    Masaaki Taniguchi

  3. Food Resources Education & Research Center, Kobe University, Kasai, 675-2103, Japan

    Kenji Oyama

Authors
  1. Shogo Hoashi
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  2. Nobuhisa Ashida
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  3. Hideki Ohsaki
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  4. Takeshi Utsugi
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  5. Shinji Sasazaki
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  6. Masaaki Taniguchi
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  7. Kenji Oyama
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  8. Fumio Mukai
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  9. Hideyuki Mannen
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Corresponding author

Correspondence to Hideyuki Mannen.

Additional information

S. Hoashi and N. Ashida contributed equally to this work.

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Hoashi, S., Ashida, N., Ohsaki, H. et al. Genotype of bovine sterol regulatory element binding protein-1 (SREBP-1) is associated with fatty acid composition in Japanese Black cattle. Mamm Genome 18, 880–886 (2007). https://doi.org/10.1007/s00335-007-9072-y

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  • DOI: https://doi.org/10.1007/s00335-007-9072-y

Keywords

  • Fatty Acid Composition
  • Sterol Regulatory Element Binding Protein
  • Holstein Cattle
  • Japanese Black Cattle
  • Cattle Carcass