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Mapping quantitative trait loci for canine hip dysplasia in German Shepherd dogs

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Abstract

Canine hip dysplasia (CHD) is a common hereditary developmental disease of the coxofemoral joints. CHD is characterized by subluxation of the femoral head and deformation of the acetabulum leading to a painful osteoarthrosis. Analyses of mode of inheritance have shown the involvement of a major gene in expression of CHD in German Shepherd dogs. Thus, a whole genome scan for quantitative trait loci (QTL) was performed in German Shepherd dogs. For this purpose 11 paternal half-sib families, including a total of 459 purebred German Shepherd dogs with sires, dams, and offspring, were genotyped for 261 microsatellites. These markers were equidistantly distributed over all 38 autosomes and the X chromosome with an average marker distance of 11.7 cM. The mean observed heterozygosity of the marker set was 50%. The CHD status for the dogs was scored according to the official rules of the Fédération Cynologique Internationale. At the genome-wide level of significance at p < 0.05, QTL for CHD were located on nine different canine chromosomes: 1, 3, 4, 8, 9, 16, 19, 26, and 33. The minimal QTL regions containing the CHD genes spanned on average 5 Mb with a range between 1 and 8.2 Mb. Chromosome-wide level of significance at p < 0.05 was found for QTL on 19 chromosomes. Further analyses can now be performed to refine these map positions of QTL already identified in German Shepherd dogs.

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Acknowledgments

This study and Yvonne Marschall were supported by the Gesellschaft zur Förderung Kynologischer Forschung e.V. (GKF), Bonn, and the Breeding Associaton of German Shepherd Dogs (Verein für Deutsche Schäferhunde e.V., SV), Augsburg. The authors are indebted to SV and Mr. Lux, managing director of the SV, for providing the data set, X rays, the official CHD scores, and blood samples. The authors are grateful to Stefan Neander for expert technical assistance.

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  1. Institute for Animal Breeding and Genetics, University of Veterinary Medicine, Bünteweg 17 p, 30559, Hannover, Germany

    Yvonne Marschall & Ottmar Distl

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  1. Yvonne Marschall
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  2. Ottmar Distl
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Correspondence to Ottmar Distl.

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Marschall, Y., Distl, O. Mapping quantitative trait loci for canine hip dysplasia in German Shepherd dogs. Mamm Genome 18, 861–870 (2007). https://doi.org/10.1007/s00335-007-9071-z

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