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Estimating the number of coding mutations in genotypic and phenotypic driven N-ethyl-N-nitrosourea (ENU) screens: revisited

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Abstract

We recently described methods for estimating the number of N-ethyl-N-nitrosourea (ENU)-induced coding mutations in phenotypic and genotypic screens. In this article we revisit these methods, clarifying their application. In particular, we focus on the difference between unconditional and conditional probabilities. We also introduce a website to assist investigators in the application of these equations (http://www.well.ox.ac.uk/enuMutRat).

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References

  • Keays DA, Clark TG, Flint J (2006) Estimating the number of coding mutations in genotypic- and phenotypic-driven N-ethyl-N-nitrosourea (ENU) screens. Mamm Genome 17(3), 230–238

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  • Quwailid MM, Hugill A, Dear N, Vizor L, Wells S, et al. (2004) A gene-driven ENU-based approach to generating an allelic series in any gene. Mamm Genome 15(8), 585–591

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Acknowledgments

The authors thank Ian Jackson for drawing to their attention the issues articulated in this note. They are most grateful for the extremely useful comments of the referees.

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Authors and Affiliations

  1. Psychiatric Genetics Laboratory, Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford, OX3 7BN, United Kingdom

    David A. Keays, Taane G. Clark, Thomas G. Campbell, John Broxholme & William Valdar

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  1. David A. Keays
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  2. Taane G. Clark
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  3. Thomas G. Campbell
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  4. John Broxholme
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  5. William Valdar
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Correspondence to David A. Keays.

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Keays, D.A., Clark, T.G., Campbell, T.G. et al. Estimating the number of coding mutations in genotypic and phenotypic driven N-ethyl-N-nitrosourea (ENU) screens: revisited. Mamm Genome 18, 123–124 (2007). https://doi.org/10.1007/s00335-006-0065-z

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  • DOI: https://doi.org/10.1007/s00335-006-0065-z

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