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Quantitative trait loci for individual adipose depot weights in C57BL/6ByJ x 129P3/J F2 mice

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Abstract

To understand how genotype influences fat patterning and obesity, we conducted an autosomal genome scan using male and female F2 hybrids between the C57BL/6ByJ and 129P3/J parental mouse strains. Mice were studied in middle-adulthood and were fed a low-energy, low-fat diet during their lifetime. We measured the weight of the retroperitoneal adipose depot (near the kidney) and the gonadal adipose depot (near the epididymis in males and ovaries in females). An important feature of the analysis was the comparison of linkage results for absolute adipose depot weight and depot weight adjusted for body size, i.e., relative weight. We detected 67 suggestive linkages for six phenotypes, which fell into one of three categories: those specific to absolute but not relative depot weight (Chr 5, 11, and 14), those specific to relative but not absolute depot weight (Chr 9, 15, and 16), and those involving both (Chr 2 and 7). Some quantitative trait loci (QTLs) affected one adipose depot more than another: Retroperitoneal depot weight was linked to Chr 8, 11, 12, and 17, but the linkage effects for the gonadal depot were stronger for Chr 5, 7, and 9. Several linkages were specific to sex; for instance, the absolute weight of gonadal fat was linked to Chromosome 7 in male (LOD = 3.4) but not female mice (LOD = 0.2). Refining obesity as a phenotype may uncover clues about gene function that will assist in positional cloning efforts.

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Acknowledgments

Grants from the National Institutes of Health funded this research (R01DK 058797 to DRR, R01AA011028 and R01DC00882 to AAB, and R01DK046791 and R01AA12715 to MGT). The authors acknowledge Dr. Kelly Ewen-White and Paige Stevenson at the Genotyping Section at the Australian Genome Research Facility for part of the genotyping. Maria Theodorides provided excellent technical assistance. Early data collection for this experiment was conducted in the laboratory of R. Arlen Price, and his support is gratefully acknowledged. Patricia Watson provided helpful editorial advice. Discussions with Hong Ji, Mark I. Friedman, and Caroline M. Pond enhanced the quality of this work.

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Correspondence to Danielle R. Reed.

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Reed, D.R., McDaniel, A.H., Li, X. et al. Quantitative trait loci for individual adipose depot weights in C57BL/6ByJ x 129P3/J F2 mice. Mamm Genome 17, 1065–1077 (2006). https://doi.org/10.1007/s00335-006-0054-2

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  • DOI: https://doi.org/10.1007/s00335-006-0054-2

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