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Mammalian Genome

, Volume 17, Issue 6, pp 584–597 | Cite as

High resolution mapping of chromosomal regions controlling resistance to gastrointestinal nematode infections in an advanced intercross line of mice

  • Jerzy M. BehnkeEmail author
  • Fuad A. Iraqi
  • John M. Mugambi
  • Simon Clifford
  • Sonal Nagda
  • Derek Wakelin
  • Stephen J. Kemp
  • R. Leyden Baker
  • John P. Gibson
Article

Abstract

Fine mapping of quantitative trait loci (QTL) associated with resistance to the gastrointestinal parasite Heligmosomoides polygyrus was achieved on F6/F7 offspring (1076 mice) from resistant (SWR) and susceptible (CBA) mouse strains by selective genotyping (top and bottom 20% selected on total worm count in week 6). Fecal egg counts were recorded at weeks 2, 4, and 6, and the average was also analyzed. Blood packed cell volume in weeks 3 and 6 and five immunological traits (mucosal mast cell protease 1, granuloma score, IgG1 against adult worm, IgG1, and IgE to L4 antigen) were also recorded. On Chromosome 1 single-trait analyses identified a QTL with effects on eight traits located at about 24 cM on the F2 mouse genome database (MGD) linkage map, with a 95% confidence interval (CI) of 20-32 cM established from a multitrait analysis. On Chromosome 17 a QTL with effects on nine traits was located at about 18 cM on the MGD map (CI 17.9-18.4 cM). Strong candidate genes for the QTL position on Chromosome 1 include genes known to be involved in regulating immune responses and on Chromosome 17 genes within the MHC, notably the Class II molecules and tumor necrosis factor.

Keywords

Quantitative Trait Locus Quantitative Trait Locus Effect Significant Quantitative Trait Locus Quantitative Trait Locus Location Single Quantitative Trait Locus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors acknowledge the technical inputs in the helminthology laboratory of Sam Njomo, Fredrick Moseti, and Sarah Kanyingi; mouse husbandry by Jane Ikanyi, John Kiragu, and Pauline Mbuthia under the supervision of Bob King; and John Wambugu, Moses Ogugo, Daniel Mwangi, and Nemuel Nyamweya for the genotyping in the molecular genetics laboratory. The authors are particularly grateful to Clare Kemp for preparing the color versions of Figs. 2 and 3. This research was funded by a grant from the Wellcome Trust (063810) and by member donors of the CGIAR, and by program-restricted grants to ILRI from the EU and Department for International Development (DfID), UK. Helpful advice was generously provided by Prof. A.B. Korol and Prof. Jan Bradley.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Jerzy M. Behnke
    • 1
    Email author
  • Fuad A. Iraqi
    • 2
  • John M. Mugambi
    • 2
  • Simon Clifford
    • 1
  • Sonal Nagda
    • 2
  • Derek Wakelin
    • 1
  • Stephen J. Kemp
    • 3
  • R. Leyden Baker
    • 2
    • 5
  • John P. Gibson
    • 4
  1. 1.School of Biological SciencesUniversity of NottinghamNottinghamUK
  2. 2.International Livestock Research Institute (ILRI)NairobiKenya
  3. 3.School of Biological SciencesUniversity of LiverpoolLiverpoolUK
  4. 4.The Institute for Genetics and BioinformaticsUniversity of New EnglandArmidaleAustralia
  5. 5.WhangamataNew Zealand

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