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DNA polymorphisms of the prion doppel gene region in four different German cattle breeds and cows tested positive for bovine spongiform encephalopathy

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Abstract

Polymorphisms of the prion protein gene PRNP have been shown to influence the susceptibility/resistance to prion infections in human and sheep. In addition, the T174M polymorphism within the flanking prion doppel gene (PRND) was thought to be involved in susceptibility to sporadic Creutzfeldt-Jacob disease. To study a possible influence of DNA polymorphisms of the bovine PRND gene in bovine spongiform encephalopathy (BSE), previously identified and newly isolated DNA polymorphisms were genotyped in all available German cattle that tested positive for BSE. Genotypes and calculated haplotypes were compared with breeding bulls serving as controls. Analysis of the four major breeds Schwarzbunt (Holstein Friesian), Rotbunt (Holstein Red), Fleckvieh (Simmental), and Braunvieh (Swiss Brown) resulted in the isolation of the previously known polymorphisms R50H and R132Q and two novel synonymous single nucleotide polymorphisms (SNPs) C4820T and A5063T. Comparative genotype and haplotype analysis of BSE and control animals revealed a significantly different distribution of polymorphisms C4815T and R132Q in Fleckvieh animals but not in the other breeds tested. No association to BSE susceptibility was detectable for polymorphisms R50H and A5063T.

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Acknowledgments

The authors thank M.H. Groschup, U. Ziegler, and colleagues at the Friedrich-Löffler-Institut, Insel Riems (Germany) for collection of BSE tissue and DNA preparation. For the donation of bovine sperm, the authors are grateful to C. Leiding, Besamungsverein (Neustadt/Aisch, Germany), D. Fresen, Rinder- produktion Niedersachsen (Verden, Germany), W. Schütz, Rinderbesamungsstation (Memmingen, Germany), and B. Wollgarten, Top Genetic (Greifenberg, Germany). They thank B. Orlicz-Welcz and P. Wenzeler for excellent technical assistance. K. Becker, S. Seeber, and P. Strissel are acknowledged for critical reading of the manuscript. This work was supported by a grant from the Bayerisches Staatsministerium für Umwelt, Gesundheit und Verbraucherschutz as part of the Bayerischer BSE-Forschungsverbund (Erl-2) and the DFG (Schi 451/5-2).

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Authors and Affiliations

  1. Institut für Biochemie, Emil-Fischer-Zentrum, Universität Erlangen-Nürnberg, Fahrstrasse 17, 91054 , Erlangen, Germany

    N. Balbus, A. Humeny, K. Kashkevich, I. Henz, C.-M. Becker & K. Schiebel

  2. Institut für Humangenetik, Universität Heidelberg, Im Neuenheimer Feld 366, 69120, Heidelberg, Germany

    C. Fischer

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  1. N. Balbus
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  2. A. Humeny
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  3. K. Kashkevich
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  4. I. Henz
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  5. C. Fischer
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  6. C.-M. Becker
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  7. K. Schiebel
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Correspondence to K. Schiebel.

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Balbus, N., Humeny, A., Kashkevich, K. et al. DNA polymorphisms of the prion doppel gene region in four different German cattle breeds and cows tested positive for bovine spongiform encephalopathy. Mamm Genome 16, 884–892 (2005). https://doi.org/10.1007/s00335-005-0052-9

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  • DOI: https://doi.org/10.1007/s00335-005-0052-9

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