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Distinct QTLs are linked to cardiac left ventricular mass in a sex-specific manner in a normotensive inbred rat intercross

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Abstract

Genetic mapping of the progeny of an F2 intercross between WKY and WKHA rats had previously allowed us to detect male-specific linkage between locus Cm24 and left ventricular mass index (LVMI). By further expanding that analysis, we detected additional loci that were all linked to LVMI in a sex-specific manner despite their autosomal location. In males, we detected one additional locus (Lvm8) on Chromosome 5 (LOD = 3.4), the two loci Lvm13 (LOD = 4.5) and Lvm9 (LOD = 2.8) on Chromosome 17, and locus Lvm10 (LOD = 4.2) on Chromosome 12. The locus Lvm13 had the same boundaries as locus Cm26 previously reported by others using a different cross. None of these loci showed linkage to LVM in females. In contrast, we identified in females the novel locus Lvm11 on Chromosome 15 (LOD = 2.8) and locus Lvm12 (LOD = 2.7) that had the same boundaries on Chromosome 3 as locus Cm25 detected previously by others using a cross of other normotensive strains. In prepubertal males, there were no differences in the width of cardiomyocytes from WKY and WKHA rats, but cardiomyocytes from WKHA became progressively wider than that of WKY as sexual maturation progressed. Altogether, these results provide evidence that distinct genes may influence LVMI of rats in a sex-dependent manner, maybe by involving sex-specific interactions of sex steroids with particular genes involved in the determination of LVMI and/or cardiomyocyte width.

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Acknowledgments

This work was supported by the NIH/NHLBI grant HL69122 (to CFD) and by a Group Grant of the Canadian Institutes for Health Research (CIHR) to the IRCM Multidisciplinary Research Group in Hypertension.

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  1. Experimental Cardiovascular Biology Research Unit, Institut de Recherches Cliniques de Montréal (IRCM), 110 Pine Ave. West, Montreal, Quebec, Canada, H2W 1R7

    Bastien Llamas, Zhibin Jiang, Marie-Line Rainville, Sylvie Picard & Christian F. Deschepper

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  1. Bastien Llamas
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Correspondence to Christian F. Deschepper.

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Llamas, B., Jiang, Z., Rainville, ML. et al. Distinct QTLs are linked to cardiac left ventricular mass in a sex-specific manner in a normotensive inbred rat intercross. Mamm Genome 16, 700–711 (2005). https://doi.org/10.1007/s00335-005-0041-z

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