Abstract
The mouse, rat, and human MASP2 loci are situated on syntenic chromosome regions and are highly conserved. They comprise the genes for MASP-2/MAp19, TAR DNA binding protein of 43 kDa, FRAP kinase, CDT6, Polymyositis–Scleroderma 100-kDa autoantigen, spermidine synthase, and TERE which were analyzed by annotation of available gene transcript data and cross-species comparison of available genomic sequences. The human and rat genes for spermidine synthase have an additional intron compared to the mouse gene. The mouse and rat genes for Polymyositis–Scleroderma 100-kDa autoantigen have an additional exon compared to the human gene. We find support for the hypothesis that the MAp19-specific exon within the MASP2 gene may have originated in a transposable element. Blocks of highly conserved intronic sequences were found in the MASP2 gene and the TARDBP gene. The expression of all genes within the MASP2 locus was analyzed in mouse and rat. The restricted expression of MASP-2 and MAp19 mRNA in liver contrasts with the ubiquitous expression of all neighboring genes studied.
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Acknowledgments
We thank Jürgen Kraus and Michael Speicher from the Institute of Anthropology, Ludwig-Maximilians-University, Munich, for their mapping of the CDT6 gene by FISH analysis; Silke Roscher for technical assistance; and the Sanger Institute mouse sequencing groups. We are grateful to Dr. Ian C. Eperon, Department of Biochemistry, University of Leicester, for valuable discussions. The work was supported by the Wellcome Trust (grant No. 060574) and the German Research Foundation (Deutsche Forschungsgemeinschaft, STO 430/1-1, 2 and Schwerpunkt Innate Immunity).
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Stover, C.M., Lynch, N.J., Hanson, S.J. et al. Organization of the MASP2 locus and its expression profile in mouse and rat. Mamm Genome 15, 887–900 (2004). https://doi.org/10.1007/s00335-004-3006-8
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DOI: https://doi.org/10.1007/s00335-004-3006-8